Primary Antibodies

KO-Validated Antibodies

Target specificity verified against knockout cell models for reliable, reproducible data

Knockout (KO) validation is one of the most trusted ways to check if an antibody works as intended. Each antibody in this range has been tested against a model where the target gene has been switched off and no signal in that knockout sample means the antibody is only picking up your protein of interest.

St John's Laboratory lists over 700 KO-validated antibodies, tested against knockout cell lines to confirm target specificity and make experimental results easier to reproduce. By comparing antibody binding between wild-type and knockout lysates or tissues, researchers receive definitive confirmation of antibody fidelity.

Category benefits

Why choose KO-validated antibodies?

✓ Tested against knockout models
Each antibody has been tested where the protein-coding gene has been fully switched off, delivering a true negative control.
✓ High target specificity
The complete absence of signal in the knockout sample confirms the antibody is detecting your intended target and eliminates non-specific cross-reactivity.
✓ Consistent, reproducible performance
Built to deliver reproducible, batch-to-batch consistency so you spend less time troubleshooting unclear results and more time generating data.
✓ Monoclonal and polyclonal formats
Our range includes 392 monoclonal options for consistent single-epitope specificity and 351 polyclonal options for enhanced signal detection across multiple epitopes.
Where they're used

Validated applications

✓ Western Blotting (WB)
Ideal for verifying target band specificity by comparing signal intensities directly between wild-type and knockout whole-cell lysates.
✓ Immunohistochemistry (IHC) & Immunofluorescence (IF)
Confirms localized subcellular and tissue-specific staining while verifying the absence of background in knockout tissue sections.
✓ Flow Cytometry (FC / FACS)
Ensures distinct surface or intracellular target detection and accurate population gating without cross-reactive shifts.
✓ Immunoprecipitation (IP) & ELISA
Guarantees high-confidence target pull-down and quantitative capture with zero non-specific binding from background proteins.

Need help choosing a KO-validated antibody?

Contact our technical support team for help finding an antibody for your target of interest. Each product page includes a downloadable datasheet and SDS, with dispatch on overnight priority shipping with gel ice packs.

Looking for more primary antibodies?

Our primary antibody range spans monoclonal and polyclonal antibodies across multiple host species and validation methods.

 Browse primary antibodies

For Research Use Only. Not for use in diagnostic procedures.

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