• Western blot analysis of extracts of various cell lines, using CHCHD2 antibody (STJ119068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts from normal (control) and CHCHD2 knockout (KO) 293T cells, using CHCHD2 antibody (STJ119068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using [KO Validated] CHCHD2 rabbit polyclonal antibody (STJ119068) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Rat ovary using [KO Validated] CHCHD2 rabbit polyclonal antibody (STJ119068) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] CHCHD2 rabbit polyclonal antibody (STJ119068) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using CHCHD2 antibody (STJ119068) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using CHCHD2 antibody (STJ119068) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using CHCHD2 antibody (STJ119068) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CHCHD2 antibody (75-145) (STJ119068)

SKU:
STJ119068

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHCHD2 (75-145) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHCHD2
Gene ID: 51142
Uniprot ID: CHCH2_HUMAN
Immunogen Region: 75-145
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 75-145 of human CHCHD2 (NP_057223.1).
Immunogen Sequence: TLGHAITGGFSGGSNAEPAR PDITYQEPQGTQPAQQQQPC LYEIKQFLECAQNQGDIKLC EGFNEVLKQCR
Function Transcription factor. Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions (4% oxygen), as well as normoxia conditions (20% oxygen).
Protein Name Coiled-Coil-Helix-Coiled-Coil-Helix Domain-Containing Protein 2
Aging-Associated Gene 10 Protein
Hcv Ns2 Trans-Regulated Protein
Ns2tp
Database Links Reactome: R-HSA-1268020
Cellular Localisation Nucleus
Mitochondrion
Mitochondrion Intermembrane Space
Mainly Localized In The Intermembrane Space
Alternative Antibody Names Anti-Coiled-Coil-Helix-Coiled-Coil-Helix Domain-Containing Protein 2 antibody
Anti-Aging-Associated Gene 10 Protein antibody
Anti-Hcv Ns2 Trans-Regulated Protein antibody
Anti-Ns2tp antibody
Anti-CHCHD2 antibody
Anti-C7orf17 antibody
Anti-AAG10 antibody

Information sourced from Uniprot.org

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