Conjugated Secondary Antibodies | St John's Laboratory

Immunofluorescence – anti-mCherry antibody (STJ140145) using hCEC cells transduced with mCherry-Rab5a; cells were fixed with methanol and mCherry antibody at 1/250; 2nd antibody goat anti-IgY (STJ140242) at 1:1,000

Chicken Anti-Chicken IgY (Full length) antibody {DyLight®405} (STJ140242)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IF

Reactivity :

Chicken

£92.50 - £412.50

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Chicken Anti-Chicken IgY (Full length) antibody {DyLight®488} (STJ140243)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IF

Reactivity :

Chicken

£92.50 - £412.50

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Chicken Anti-Chicken IgY (Full length) antibody {DyLight®550} (STJ140244)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IF

Reactivity :

Chicken

£92.50 - £412.50

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Chicken Anti-Chicken IgY (Full length) antibody {DyLight®633} (STJ140245)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IF

Reactivity :

Chicken

£92.50 - £412.50

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Immunofluorescence – anti-mCherry antibody using COS7 cells transfected with mCherry-TUB1B; cells were fixed with methanol, 1st antibody anti-mCherry at 1/250 and 2nd antibody anti-goat IgY conjugated to DyLight®405 at 1/500

Chicken Anti-Goat IgG (Full length) antibody {DyLight®405} (STJ140221)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IF

Reactivity :

Goat

£92.50 - £412.50

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Immunofluorescence in Drosophila larvae NMJ muscle 6/7 expressing mCherry in neurons (DyGlutmcherry) using 1st antibody anti-mCherry at 1:1,000 and 2nd antibody anti-goat IgY conjugated to DyLight®488 at 1/500

Chicken Anti-Goat IgG (Full length) antibody {DyLight®488} (STJ140222)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IF

Reactivity :

Goat

£92.50 - £412.50

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Anti-GAPDH antibody at 1/2, 500 dilution using HEK293 transfected cell lysates at 50 µg per lane; chicken polyclonal to goat IgG conjugated to DyLight® 550 (STJ140223) at 1:10000 dilution

Chicken Anti-Goat IgG (Full length) antibody {DyLight®550} (STJ140223)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IF

Reactivity :

Goat

£92.50 - £412.50

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Chicken Anti-Goat IgG (Full length) antibody {DyLight®633} (STJ140224)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IF

Reactivity :

Goat

£92.50 - £412.50

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Western blot using using lysates at 30 µg per lane; 1st antibody anti-GAPDH antibody (STJ140037) at 1:2, 500 and 2nd antibody anti-goat conjugated to HRP (STJ140211) at 1:10000

Chicken Anti-Goat IgG antibody {HRP} (STJ140211)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/ELISA

Reactivity :

Goat

£69.50 - £412.50

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Chicken Anti-Goat IgG antibody {HRP} (STJS001245)

Clonality :

Polyclonal

Host :

Chicken

Applications :

IHC/ICC/WB

Reactivity :

Goat

£329.50

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Chicken Anti-Human IgG antibody {FITC} (STJS001247)

Clonality :

Polyclonal

Host :

Chicken

Applications :

IHC/ICC/WB

Reactivity :

Human

£287.50

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Chicken Anti-Mouse IgG antibody {FITC} (STJS001249)

Clonality :

Polyclonal

Host :

Chicken

Applications :

IHC/ICC/WB

Reactivity :

Mouse

£286.50

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Chicken Anti-Mouse IgG antibody {HRP} (STJS001250)

Clonality :

Polyclonal

Host :

Chicken

Applications :

IHC/ICC/WB

Reactivity :

Mouse

£329.50

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Chicken Anti-Rabbit IgG antibody {FITC} (STJS001251)

Clonality :

Polyclonal

Host :

Chicken

Applications :

IHC/ICC/WB

Reactivity :

Rabbit

£287.50

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Chicken Anti-Rabbit IgG antibody {HRP} (STJS001252)

Clonality :

Polyclonal

Host :

Chicken

Applications :

IHC/ICC/WB

Reactivity :

Rabbit

£329.50

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Donkey Anti-Goat IgG (H&L) antibody {Alexa Fluor 488} (STJS000807)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Goat

£208.50

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Donkey Anti-Goat IgG (H&L) antibody {Alexa Fluor 555} (STJS000798)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Goat

£208.50

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Donkey Anti-Goat IgG (H&L) antibody {Alexa Fluor 568} (STJS000816)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Goat

£208.50

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Donkey Anti-Goat IgG (H&L) antibody {Alexa Fluor 594} (STJS000825)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Goat

£208.50

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Donkey Anti-Goat IgG (H&L) antibody {Alexa Fluor 633} (STJS000834)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Goat

£208.50

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Donkey Anti-Goat IgG (H&L) antibody {Alexa Fluor 647} (STJS000844)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Goat

£208.50

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Donkey Anti-Goat IgG H+L antibody {FITC} (STJS001181)

Clonality :

Polyclonal

Host :

Donkey

Applications :

IF/ICC

Reactivity :

Goat

£67.50 - £112.50

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Donkey Anti-Goat IgG H+L antibody {HRP} (STJS001180)

Clonality :

Polyclonal

Host :

Donkey

Applications :

WB/IHC-P

Reactivity :

Goat

£51.50 - £79.50

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Donkey Anti-Mouse IgG (H&L) antibody {Alexa Fluor 633} (STJS000835)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Mouse

£208.50

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Western blot analysis of recombinant Mouse control IgG Protein using HRP Donkey Anti-Mouse IgG (H+L) (STJS001182) at 1:10000 dilution. <br/>Secondary antibody:HRP Donkey Anti-Mouse IgG (H+L) (STJS001182) at 1:10000 dilution. <br/>Lysates/proteins: 100ng/50ng per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 20s. <br/><br/>

Donkey Anti-Mouse IgG H+L antibody {HRP} (STJS001182)

Clonality :

Polyclonal

Host :

Donkey

Applications :

WB/IHC-P

Reactivity :

Mouse

£51.50 - £79.50

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Donkey Anti-Mouse IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 488} (STJS000808)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Mouse

£208.50

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Donkey Anti-Mouse IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 555} (STJS000799)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Mouse

£208.50

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Donkey Anti-Mouse IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 568} (STJS000817)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Mouse

£208.50

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Donkey Anti-Mouse IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 594} (STJS000826)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Mouse

£208.50

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Donkey Anti-Mouse IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 647} (STJS000845)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Mouse

£208.50

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Donkey Anti-Rabbit IgG (H&L) antibody {Alexa Fluor 633} (STJS000836)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Rabbit

£208.50

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Immunofluorescence analysis of PC-12 cells using Vimentin Rabbit mAb (STJ1161657) at a dilution of 1:100 (40x lens). Secondary antibody: FITC Donkey Anti-Rabbit IgG (H+L) (STJS001188) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Donkey Anti-Rabbit IgG H+L antibody {FITC} (STJS001188)

Clonality :

Polyclonal

Host :

Donkey

Applications :

IF/ICC/FC

Reactivity :

Rabbit

£67.50 - £112.50

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Western blot analysis of lysates from HeLa cells, using Beta-actin antibody as the primary antibody. <br/>Secondary antibody: HRP Donkey Anti-Rabbit IgG (H+L) antibody (STJS001185) at 1:2000-1:30000 dilution<br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Donkey Anti-Rabbit IgG H+L antibody {HRP} (STJS001185)

Clonality :

Polyclonal

Host :

Donkey

Applications :

WB/IHC-P

Reactivity :

Rabbit

£51.50 - £79.50

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Donkey Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 488} (STJS000809)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Rabbit

£208.50

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Donkey Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 555} (STJS000800)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Rabbit

£208.50

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Donkey Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 568} (STJS000818)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Rabbit

£208.50

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Donkey Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 594} (STJS000827)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Rabbit

£208.50

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Donkey Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 647} (STJS000846)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Rabbit

£208.50

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Donkey Anti-Rat IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 594} (STJS000828)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Rat

£208.50

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Goat Anti-Chicken IgY (IgG) Heavy & Light Chain antibody {Biotin} (STJ99422)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC/IF/ELISA

Reactivity :

Chicken

£129.00

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Goat Anti-Chicken IgY antibody {Biotin} (STJS001243)

Clonality :

Polyclonal

Host :

Goat

Applications :

IHC/ICC/WB

Reactivity :

Chicken

£368.50

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Goat Anti-Chicken IgY antibody {FITC} (STJS001244)

Clonality :

Polyclonal

Host :

Goat

Applications :

IHC/ICC/WB

Reactivity :

Chicken

£279.50

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To use the Alexa Fluors with fluorescent imagers, use a spectral line of the blue laser diode for Alexa Fluors 405, a cyan (488 nm) laser for Alexa Fluors 488, a yellow (526 nm) laser for Alexa Fluor 550 or 594, and a red (633 nm) laser for Alexa Fluor 649. The Alexa Fluor 680 and 790 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm, and 800 nm

Goat Anti-Chicken IgY Heavy & Light Chain antibody {Alexa Fluor 350} (STJS000208)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IF/FC

Reactivity :

Chicken

£169.00 - £1,061.00

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Goat Anti-Chicken IgY Heavy & Light Chain antibody {Alexa Fluor 405} (STJS000251)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IF/FC

Reactivity :

Chicken

£169.00 - £1,061.00

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Goat Anti-Chicken IgY Heavy & Light Chain antibody {Alexa Fluor 488} (STJS000294)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IF/FC

Reactivity :

Chicken

£169.00 - £1,061.00

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Goat Anti-Chicken IgY Heavy & Light Chain antibody {Alexa Fluor 532} (STJS000337)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IF/FC

Reactivity :

Chicken

£169.00 - £1,061.00

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Goat Anti-Chicken IgY Heavy & Light Chain antibody {Alexa Fluor 555} (STJS000380)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IF/FC

Reactivity :

Chicken

£169.00 - £1,061.00

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Goat Anti-Chicken IgY Heavy & Light Chain antibody {Alexa Fluor 568} (STJS000423)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IF/FC

Reactivity :

Chicken

£169.00 - £1,061.00

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Conjugation