![Western blot analysis of lysates from wild type (WT) and CHD1 knockout (KO) 293T cells, using [KO Validated] CHD1 Rabbit pAb (STJ110193) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/88512/404526/STJ110193_1__90370.1744890209.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CHD1 knockout (KO) 293T cells, using [KO Validated] CHD1 Rabbit pAb (STJ110193) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.")
![Western blot analysis of lysates from Jurkat cells, using [KO Validated] CHD1 Rabbit pAb (STJ110193) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/88512/404527/STJ110193_2__02217.1744890210.jpg?c=1 "Western blot analysis of lysates from Jurkat cells, using [KO Validated] CHD1 Rabbit pAb (STJ110193) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.")
![Immunohistochemistry analysis of paraffin-embedded Mouse brain using [KO Validated] CHD1 Rabbit pAb (STJ110193) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/88512/404528/STJ110193_3__12723.1744890210.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded Mouse brain using [KO Validated] CHD1 Rabbit pAb (STJ110193) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of paraffin-embedded Human liver damage using [KO Validated] CHD1 Rabbit pAb (STJ110193) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/88512/404529/STJ110193_4__35726.1744890211.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded Human liver damage using [KO Validated] CHD1 Rabbit pAb (STJ110193) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of paraffin-embedded Rat brain using [KO Validated] CHD1 Rabbit pAb (STJ110193) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/88512/404530/STJ110193_5__18652.1744890211.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded Rat brain using [KO Validated] CHD1 Rabbit pAb (STJ110193) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.")
![Western blot analysis of lysates from wild type (WT) and CHD1 knockout (KO) 293T cells, using [KO Validated] CHD1 Rabbit pAb (STJ110193) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/88512/404526/STJ110193_1__90370.1744890209.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CHD1 knockout (KO) 293T cells, using [KO Validated] CHD1 Rabbit pAb (STJ110193) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.")
Anti-CHD1 antibody (C-Term) (STJ110193)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CHD1 (C-Term) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:100-1:500IHC-P:1:100-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CHD1 |
| Gene ID | 1105 |
| Uniprot ID | CHD1_HUMAN |
| Immunogen Region | C-Term |
| Immunogen Sequence | DREKHRKLDDHRSRDHRSNL EGSLKDRSHSDHRSHSDHRL HSDHRSSSEYTHHKSSRDYR YHSDWQMDHRASSSGPRSPL DQRSPYGSRSPFEHSVEHKS TPEHTWSSRKT |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1600 to the C-terminus of human CHD1 (NP_001261.2). |
Additional Info
| Tissue Specificity | Expressed in many tissues including in the brain, where the highest level of expression is found in the cerebellum and basal ganglia. |
| Function | ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Is also associated with histone deacetylase (HDAC) activity. Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. Required for maintaining open chromatin and pluripotency in embryonic stem cells. |
| Protein Name | Chromodomain-Helicase-Dna-Binding Protein 1Chd-1Atp-Dependent Helicase Chd1 |
| Database Links | Reactome: R-HSA-9018519 |
| Cellular Localisation | NucleusCytoplasmIs Released Into The Cytoplasm When Cells Enter Mitosis And Is Reincorporated Into Chromatin During Telophase-Cytokinesis |
| Alternative Antibody Names | Anti-Chromodomain-Helicase-Dna-Binding Protein 1 antibodyAnti-Chd-1 antibodyAnti-Atp-Dependent Helicase Chd1 antibodyAnti-CHD1 antibody |
Information sourced from Uniprot.org