• Western blot analysis of extracts from normal (control) and CHD1 knockout (KO) 293T cells, using CHD1 antibody (STJ110193) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using CHD1 antibody (STJ110193) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Jurkat cells, using CHD1 antibody (STJ110193) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human liver damage using CHD1 antibody (STJ110193) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using CHD1 antibody (STJ110193) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using CHD1 antibody (STJ110193) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver damage using CHD1 antibody (STJ110193) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of Jurkat cells, using CHD1 antibody (STJ110193) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded rat brain using CHD1 antibody (STJ110193) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts from normal (control) and CHD1 knockout (KO) 293T cells, using CHD1 antibody (STJ110193) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHD1 antibody (C-Term) (STJ110193)

SKU:
STJ110193

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHD1 (C-Term) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHD1
Gene ID: 1105
Uniprot ID: CHD1_HUMAN
Immunogen Region: C-Term
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1600 to the C-terminus of human CHD1 (NP_001261.2).
Immunogen Sequence: DREKHRKLDDHRSRDHRSNL EGSLKDRSHSDHRSHSDHRL HSDHRSSSEYTHHKSSRDYR YHSDWQMDHRASSSGPRSPL DQRSPYGSRSPFEHSVEHKS TPEHTWSSRKT
Tissue Specificity Expressed in many tissues including in the brain, where the highest level of expression is found in the cerebellum and basal ganglia.
Function ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Is also associated with histone deacetylase (HDAC) activity. Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. Required for maintaining open chromatin and pluripotency in embryonic stem cells.
Protein Name Chromodomain-Helicase-Dna-Binding Protein 1
Chd-1
Atp-Dependent Helicase Chd1
Database Links Reactome: R-HSA-9018519
Cellular Localisation Nucleus
Cytoplasm
Is Released Into The Cytoplasm When Cells Enter Mitosis And Is Reincorporated Into Chromatin During Telophase-Cytokinesis
Alternative Antibody Names Anti-Chromodomain-Helicase-Dna-Binding Protein 1 antibody
Anti-Chd-1 antibody
Anti-Atp-Dependent Helicase Chd1 antibody
Anti-CHD1 antibody

Information sourced from Uniprot.org

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