Tag antibodies target short peptide sequences fused to a protein of interest, giving researchers a way to detect, purify or track recombinant proteins without needing a separate antibody for each native target.
Tag antibodies are raised against short peptide epitopes (typically 6–15 amino acids) that are genetically fused to recombinant proteins. Common tags include His‑tag, HA‑tag, FLAG‑tag, Myc‑tag, and GST‑tag. These antibodies enable researchers to detect fusion proteins using standard immunoassay techniques like Western blotting, immunoprecipitation, immunofluorescence, and ELISA without the need for target‑specific antibodies.
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Versatile detection across multiple targets A single tag antibody can detect any recombinant protein carrying the corresponding tag, eliminating the need to generate or source target‑specific antibodies for each new protein of interest. |
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High specificity and affinity Tag antibodies are affinity‑purified against the epitope peptide, delivering low background and high signal‑to‑noise ratios in immunoassays. |
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Cost‑effective alternative By using a single tag antibody to study many different recombinant proteins, laboratories can reduce antibody inventory costs and simplify experimental design. |
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Western blotting Tag antibodies are widely used to confirm the expression and molecular weight of recombinant fusion proteins in cell lysates, providing a direct readout of protein production and integrity. |
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Immunoprecipitation (IP) Tag antibodies enable efficient pull‑down of tagged proteins from complex mixtures, making them essential tools for studying protein–protein interactions, post‑translational modifications, and protein complex composition. |
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Immunofluorescence and IHC Tag antibodies are used to localise recombinant proteins within cells or tissues, revealing subcellular distribution and trafficking patterns of tagged fusion proteins. |
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Affinity purification Tag antibodies immobilised on solid supports are used in affinity chromatography to purify tagged recombinant proteins from cell lysates or culture supernatants with high yield and purity. |
Our scientific support team is available to help you select the optimal product for your specific application and experimental conditions. Contact us for expert guidance on tag choice, antibody validation, and assay development.
Looking for more primary antibodies?Explore our full range of tag antibodies for reliable detection and purification of recombinant proteins. |
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Clonality :
Monoclonal
Host :
Mouse
Applications :
ELISA/WB
Reactivity :
General