• Immunohistochemistry analysis of COX5A in paraffin-embedded human lung cancer tissue using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of COX5A in paraffin-embedded human liver cancer tissue using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of COX5A in paraffin-embedded mouse kidney tissue using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of COX5A in paraffin-embedded human colon carcinoma tissue using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of COX5A in paraffin-embedded rat brain tissue using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from wild type (WT) and COX5A knockdown (KD) HeLa (KD) cells, using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of various lysates, using [KO Validated] COX5A Rabbit polyclonal antibody (STJ11105366) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-COX5A antibody (1-150) (STJ11105366)

SKU:
STJ11105366

£134.50 - £666.50
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cytochrome C Oxidase Subunit 5a-Mitochondrial (1-150) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COX5A
Gene ID: 9377
Uniprot ID: COX5A_HUMAN
Immunogen Region: 1-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human COX5A (NP_004246.2).
Immunogen Sequence: MLGAALRRCAVAATTRADPR GLLHSARTPGPAVAIQSVRC YSHGSQETDEEFDARWVTYF NKPDIDAWELRKGINTLVTY DMVPEPKIIDAALRACRRLN DFASTVRILEVVKDKAGPHK EIYPYVIQELRPTLNELGIS TPEELGLDKV
Function Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Protein Name Cytochrome C Oxidase Subunit 5a - Mitochondrial
Cytochrome C Oxidase Polypeptide Va
Database Links Reactome: R-HSA-5628897
Reactome: R-HSA-611105
Reactome: R-HSA-9707564
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Cytochrome C Oxidase Subunit 5a - Mitochondrial antibody
Anti-Cytochrome C Oxidase Polypeptide Va antibody
Anti-COX5A antibody

Information sourced from Uniprot.org

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