Biotinylated secondary antibodies

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Biotin-conjugated secondary antibodies

Biotin, also known as Vitamin H or B7, is an essential part of normal body functions, including the metabolisation of different amino acids, fats and carbohydrates, and is commonly found in salmon, liver, dairy products, eggs and other foods.


The process of conjugating this substance to a secondary antibody is named biotinylation and is relatively fast and simple due to the small size of the molecule and the fact that it doesn't have a significant impact on the antibody's function. 



Biotinylated secondary antibodies are often used together with streptavidin or avidin-based conjugates to generate signal amplification in assays like ELISA, immunofluorescence or western blot, where accurate detection of low-abundance proteins is needed, and they can also be utilised in different protein purification methods. 

Browse our wide selection of high-quality biotinylated secondary antibodies.