• Immunofluorescence analysis of HeLa cells using [KO Validated] CSNK1E Rabbit polyclonal antibody (STJ28096). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and CSNK1E knockout (KO) HeLa cells, using [KO Validated] CSNK1E Rabbit polyclonal antibody (STJ28096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of various lysates using [KO Validated] CSNK1E Rabbit polyclonal antibody (STJ28096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CSNK1E antibody (247-416) (STJ28096)

SKU:
STJ28096

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CSNK1E (247-416) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CSNK1E
Gene ID: 102800317/1454
Uniprot ID: KC1E_HUMAN
Immunogen Region: 247-416
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 247-416 of human CSNK1E (NP_689407.1).
Immunogen Sequence: EFSTYLNFCRSLRFDDKPDY SYLRQLFRNLFHRQGFSYDY VFDWNMLKFGAARNPEDVDR ERREHEREERMGQLRGSATR ALPPGPPTGATANRLRSAAE PVASTPASRIQPAGNTSPRA ISRVDRERKVSMRLHRGAPA NVSSSDLTGRQEVSRIPASQ TSVPFDHLGK
Tissue Specificity Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
Post Translational Modifications Autophosphorylated. Partially dephosphorylated by PPP5C. May be dephosphorylated by PP1.
Function Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates (Probable). Participates in Wnt signaling. Phosphorylates DVL1. Phosphorylates DVL2. Phosphorylates NEDD9/HEF1. Central component of the circadian clock. In balance with PP1, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. Controls PER1 and PER2 nuclear transport and degradation. Inhibits cytokine-induced granuloytic differentiation.
Protein Name Casein Kinase I Isoform Epsilon
Cki-Epsilon
Ckie
Database Links Reactome: R-HSA-201688
Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-400253
Reactome: R-HSA-5620912
Reactome: R-HSA-6791226
Reactome: R-HSA-8854518
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Casein Kinase I Isoform Epsilon antibody
Anti-Cki-Epsilon antibody
Anti-Ckie antibody
Anti-CSNK1E antibody

Information sourced from Uniprot.org

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