![Immunofluorescence analysis of HeLa cells using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101151/388526/STJ27455_1__36817.1713158040.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of C6 cells using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101151/388527/STJ27455_2__04678.1713158041.jpg?c=1 "Immunofluorescence analysis of C6 cells using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of U2OS cells using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101151/388528/STJ27455_3__07280.1713158042.jpg?c=1 "Immunofluorescence analysis of U2OS cells using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from 293T cells using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101151/388529/STJ27455_4__11373.1713158042.jpg?c=1 "Western blot analysis of lysates from 293T cells using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western blot analysis of various lysates using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101151/388530/STJ27455_5__06797.1713158043.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western blot analysis of lysates from wild type (WT) and DNAJB1 knockout (KO) 293T cells, using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101151/388531/STJ27455_6__10885.1713158043.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and DNAJB1 knockout (KO) 293T cells, using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunofluorescence analysis of HeLa cells using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/101151/388526/STJ27455_1__36817.1713158040.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
Anti-DNAJB1 antibody (1-340) (STJ27455)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DNAJB1 (1-340) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DNAJB1 |
| Gene ID | 3337 |
| Uniprot ID | DNJB1_HUMAN |
| Immunogen Region | 1-340 |
| Immunogen Sequence | MGKDYYQTLGLARGASDEEI KRAYRRQALRYHPDKNKEPG AEEKFKEIAEAYDVLSDPRK REIFDRYGEEGLKGSGPSGG SGGGANGTSFSYTFHGDPHA MFAEFFGGRNPFDTFFGQRN GEEGMDIDDPFSGFPMGMGG FTNVNFGRSRSAQEPARKKQ DPPVTHDLRVSLEEIYSGCT KKMKISHKRLNPDGKSIRNE DKILTIEVKKGWKEGTKITF PKEGDQTSNNIPADIVFVL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human DNAJB1 (NP_006136.1). |
Additional Info
| Function | Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response. Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro). |
| Protein Name | Dnaj Homolog Subfamily B Member 1Dnaj Protein Homolog 1Heat Shock 40 Kda Protein 1Hsp40Heat Shock Protein 40Human Dnaj Protein 1Hdj-1 |
| Database Links | Reactome: R-HSA-3371453Reactome: R-HSA-3371497Reactome: R-HSA-3371568Reactome: R-HSA-3371571Reactome: R-HSA-5687128 |
| Cellular Localisation | CytoplasmNucleusNucleolusTranslocates Rapidly From The Cytoplasm To The NucleusAnd Especially To The NucleoliUpon Heat Shock |
| Alternative Antibody Names | Anti-Dnaj Homolog Subfamily B Member 1 antibodyAnti-Dnaj Protein Homolog 1 antibodyAnti-Heat Shock 40 Kda Protein 1 antibodyAnti-Hsp40 antibodyAnti-Heat Shock Protein 40 antibodyAnti-Human Dnaj Protein 1 antibodyAnti-Hdj-1 antibodyAnti-DNAJB1 antibodyAnti-DNAJ1 antibodyAnti-HDJ1 antibodyAnti-HSPF1 antibody |
Information sourced from Uniprot.org