• Immunofluorescence analysis of HeLa cells using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from 293T cells using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of lysates from wild type (WT) and DNAJB1 knockout (KO) 293T cells, using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-DNAJB1 antibody (1-340) (STJ27455)

SKU:
STJ27455

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNAJB1 (1-340) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DNAJB1
Gene ID: 3337
Uniprot ID: DNJB1_HUMAN
Immunogen Region: 1-340
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human DNAJB1 (NP_006136.1).
Immunogen Sequence: MGKDYYQTLGLARGASDEEI KRAYRRQALRYHPDKNKEPG AEEKFKEIAEAYDVLSDPRK REIFDRYGEEGLKGSGPSGG SGGGANGTSFSYTFHGDPHA MFAEFFGGRNPFDTFFGQRN GEEGMDIDDPFSGFPMGMGG FTNVNFGRSRSAQEPARKKQ DPPVTHDLRVSLEEIYSGCT KKMKISHKRLNPDGKSIRNE DKILTIEVKKGWKEGTKITF PKEGDQTSNNIPADIVFVL
Function Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response. Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro).
Protein Name Dnaj Homolog Subfamily B Member 1
Dnaj Protein Homolog 1
Heat Shock 40 Kda Protein 1
Hsp40
Heat Shock Protein 40
Human Dnaj Protein 1
Hdj-1
Database Links Reactome: R-HSA-3371453
Reactome: R-HSA-3371497
Reactome: R-HSA-3371568
Reactome: R-HSA-3371571
Reactome: R-HSA-5687128
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Translocates Rapidly From The Cytoplasm To The Nucleus
And Especially To The Nucleoli
Upon Heat Shock
Alternative Antibody Names Anti-Dnaj Homolog Subfamily B Member 1 antibody
Anti-Dnaj Protein Homolog 1 antibody
Anti-Heat Shock 40 Kda Protein 1 antibody
Anti-Hsp40 antibody
Anti-Heat Shock Protein 40 antibody
Anti-Human Dnaj Protein 1 antibody
Anti-Hdj-1 antibody
Anti-DNAJB1 antibody
Anti-DNAJ1 antibody
Anti-HDJ1 antibody
Anti-HSPF1 antibody

Information sourced from Uniprot.org

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