• Immunohistochemistry analysis of EDF1 in paraffin-embedded mouse lung using [KO Validated] EDF1 Rabbit polyclonal antibody (STJ23470) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of EDF1 in paraffin-embedded human gastric cancer using [KO Validated] EDF1 Rabbit polyclonal antibody (STJ23470) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of EDF1 in paraffin-embedded rat brain using [KO Validated] EDF1 Rabbit polyclonal antibody (STJ23470) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and EDF1 knockout (KO) NIH/3T3 cells, using [KO Validated] EDF1 Rabbit polyclonal antibody (STJ23470) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates using [KO Validated] EDF1 Rabbit polyclonal antibody (STJ23470) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EDF1 antibody (1-148) (STJ23470)

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STJ23470

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EDF1 (1-148) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EDF1
Gene ID: 8721
Uniprot ID: EDF1_HUMAN
Immunogen Region: 1-148
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human EDF1 (NP_003783.1).
Immunogen Sequence: MAESDWDTVTVLRKKGPTAA QAKSKQAILAAQRRGEDVET SKKWAAGQNKQHSITKNTAK LDRETEELHHDRVTLEVGKV IQQGRQSKGLTQKDLATKIN EKPQVIADYESGRAIPNNQV LGKIERAIGLKLRGKDIGKP IEKGPRAK
Tissue Specificity Expressed in brain, liver, lung, kidney and heart (at protein level). Ubiquitously expressed. More abundant in heart, pancreas, liver, intestine and adipose tissues.
Post Translational Modifications Phosphorylated (by PKA and PKC).
Function Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism.
Protein Name Endothelial Differentiation-Related Factor 1
Edf-1
Multiprotein-Bridging Factor 1
Mbf1
Cellular Localisation Cytoplasm
Nucleus
Also Nuclear Upon Binding To Nr5a1 And Treatment Of Cells With Tpa Or Forskolin
Alternative Antibody Names Anti-Endothelial Differentiation-Related Factor 1 antibody
Anti-Edf-1 antibody
Anti-Multiprotein-Bridging Factor 1 antibody
Anti-Mbf1 antibody
Anti-EDF1 antibody

Information sourced from Uniprot.org

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