![Western blot analysis of lysates from wild type (WT) and CSDE1 knockout (KO) HeLa cells, using [KO Validated] CSDE1 Rabbit polyclonal antibody (STJ27737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101329/388954/STJ27737_3__92253.1713158535.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CSDE1 knockout (KO) HeLa cells, using [KO Validated] CSDE1 Rabbit polyclonal antibody (STJ27737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.")
Anti-CSDE1 antibody (369-600) (STJ27737)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CSDE1 (369-600) antibody for use in WB, IF, ICC, IP and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CSDE1 |
| Gene ID | 7812 |
| Uniprot ID | CSDE1_HUMAN |
| Immunogen Region | 369-600 |
| Immunogen Sequence | LSAQRNHAIRIKKLPKGTVS FHSHSDHRFLGTVEKEATFS NPKTTSPNKGKEKEAEDGII AYDDCGVKLTIAFQAKDVEG STSPQIGDKVEFSISDKQRP GQQVATCVRLLGRNSNSKRL LGYVATLKDNFGFIETANHD KEIFFHYSEFSGDVDSLELG DMVEYSLSKGKGNKVSAEKV NKTHSVNGITEEADPTIYSG KVIRPLRSVDPTQTEYQGMI EIVEEGDMKGEV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 369-600 of human CSDE1 (NP_009089.4). |
Additional Info
| Function | RNA-binding protein involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Required for efficient formation of stress granules. (Microbial infection) Required for internal initiation of translation of human rhinovirus RNA. |
| Protein Name | Cold Shock Domain-Containing Protein E1N-Ras Upstream Gene ProteinProtein Unr |
| Database Links | |
| Cellular Localisation | CytoplasmStress GranuleP-Body |
| Alternative Antibody Names | Anti-Cold Shock Domain-Containing Protein E1 antibodyAnti-N-Ras Upstream Gene Protein antibodyAnti-Protein Unr antibodyAnti-CSDE1 antibodyAnti-D1S155E antibodyAnti-KIAA0885 antibodyAnti-NRU antibodyAnti-UNR antibody |
Information sourced from Uniprot.org