![Western blot analysis of lysates from wild type (WT) and DNAJA1 knockout (KO) 293T cells, using [KO Validated] DNAJA1 Rabbit polyclonal antibody (STJ11100741) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89679/358172/STJ11100741_1__59623.1713122479.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and DNAJA1 knockout (KO) 293T cells, using [KO Validated] DNAJA1 Rabbit polyclonal antibody (STJ11100741) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and DNAJA1 knockout (KO) 293T cells, using [KO Validated] DNAJA1 Rabbit polyclonal antibody (STJ11100741) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89679/358172/STJ11100741_1__59623.1713122479.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and DNAJA1 knockout (KO) 293T cells, using [KO Validated] DNAJA1 Rabbit polyclonal antibody (STJ11100741) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-DNAJA1 antibody (178-397) (STJ11100741)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DNAJA1 (178-397) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DNAJA1 |
| Gene ID | 3301 |
| Uniprot ID | DNJA1_HUMAN |
| Immunogen Region | 178-397 |
| Immunogen Sequence | MECQGHGERISPKDRCKSCN GRKIVREKKILEVHIDKGMK DGQKITFHGEGDQEPGLEPG DIIIVLDQKDHAVFTRRGED LFMCMDIQLVEALCGFQKPI STLDNRTIVITSHPGQIVKH GDIKCVLNEGMPIYRRPYEK GRLIIEFKVNFPENGFLSPD KLSLLEKLLPERKEVEETDE MDQVELVDFDPNQERRRHYN GEAYEDDEHHPRGGVQCQTS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 178-397 of human DNAJA1 (NP_001530.1). |
Additional Info
| Tissue Specificity | Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver. |
| Function | Co-chaperone for HSPA8/Hsc70. Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as a co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis. Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV. |
| Protein Name | Dnaj Homolog Subfamily A Member 1Dnaj Protein Homolog 2HsdjHeat Shock 40 Kda Protein 4Heat Shock Protein J2Hsj-2Human Dnaj Protein 2Hdj-2 |
| Database Links | Reactome: R-HSA-3371497Reactome: R-HSA-9841251 |
| Cellular Localisation | MembraneLipid-AnchorCytoplasmMicrosomeNucleusPerinuclear RegionMitochondrionPrimarily Associated With MicrosomesA Minor Proportion Is Associated With MitochondriaPrimarily CytoplasmicA Minor Proportion Is Associated With Nuclei |
| Alternative Antibody Names | Anti-Dnaj Homolog Subfamily A Member 1 antibodyAnti-Dnaj Protein Homolog 2 antibodyAnti-Hsdj antibodyAnti-Heat Shock 40 Kda Protein 4 antibodyAnti-Heat Shock Protein J2 antibodyAnti-Hsj-2 antibodyAnti-Human Dnaj Protein 2 antibodyAnti-Hdj-2 antibodyAnti-DNAJA1 antibodyAnti-DNAJ2 antibodyAnti-HDJ2 antibodyAnti-HSJ2 antibodyAnti-HSPF4 antibody |
Information sourced from Uniprot.org