• Western blot analysis of lysates from wild type (WT) and DNAJA1 knockout (KO) 293T cells, using [KO Validated] DNAJA1 Rabbit polyclonal antibody (STJ11100741) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-DNAJA1 antibody (178-397) (STJ11100741)

SKU:
STJ11100741

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNAJA1 (178-397) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DNAJA1
Gene ID: 3301
Uniprot ID: DNJA1_HUMAN
Immunogen Region: 178-397
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 178-397 of human DNAJA1 (NP_001530.1).
Immunogen Sequence: MECQGHGERISPKDRCKSCN GRKIVREKKILEVHIDKGMK DGQKITFHGEGDQEPGLEPG DIIIVLDQKDHAVFTRRGED LFMCMDIQLVEALCGFQKPI STLDNRTIVITSHPGQIVKH GDIKCVLNEGMPIYRRPYEK GRLIIEFKVNFPENGFLSPD KLSLLEKLLPERKEVEETDE MDQVELVDFDPNQERRRHYN GEAYEDDEHHPRGGVQCQTS
Tissue Specificity Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver.
Function Co-chaperone for HSPA8/Hsc70. Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis. Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV.
Protein Name Dnaj Homolog Subfamily A Member 1
Dnaj Protein Homolog 2
Hsdj
Heat Shock 40 Kda Protein 4
Heat Shock Protein J2
Hsj-2
Human Dnaj Protein 2
Hdj-2
Database Links Reactome: R-HSA-3371497
Cellular Localisation Membrane
Lipid-Anchor
Cytoplasm
Microsome
Nucleus
Perinuclear Region
Mitochondrion
Primarily Associated With Microsomes
A Minor Proportion Is Associated With Mitochondria
Primarily Cytoplasmic
A Minor Proportion Is Associated With Nuclei
Alternative Antibody Names Anti-Dnaj Homolog Subfamily A Member 1 antibody
Anti-Dnaj Protein Homolog 2 antibody
Anti-Hsdj antibody
Anti-Heat Shock 40 Kda Protein 4 antibody
Anti-Heat Shock Protein J2 antibody
Anti-Hsj-2 antibody
Anti-Human Dnaj Protein 2 antibody
Anti-Hdj-2 antibody
Anti-DNAJA1 antibody
Anti-DNAJ2 antibody
Anti-HDJ2 antibody
Anti-HSJ2 antibody
Anti-HSPF4 antibody

Information sourced from Uniprot.org

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