![Immunohistochemistry analysis of Alpha-Catenin in paraffin-embedded mouse testis using [KO Validated] Alpha-Catenin Rabbit polyclonal antibody (STJ27602) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101235/388725/STJ27602_2__90326.1713158264.jpg?c=1 "Immunohistochemistry analysis of Alpha-Catenin in paraffin-embedded mouse testis using [KO Validated] Alpha-Catenin Rabbit polyclonal antibody (STJ27602) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of Alpha-Catenin in paraffin-embedded human stomach using [KO Validated] Alpha-Catenin Rabbit polyclonal antibody (STJ27602) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101235/388726/STJ27602_3__61931.1713158265.jpg?c=1 "Immunohistochemistry analysis of Alpha-Catenin in paraffin-embedded human stomach using [KO Validated] Alpha-Catenin Rabbit polyclonal antibody (STJ27602) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of Alpha-Catenin in paraffin-embedded human vermiform appendix using [KO Validated] Alpha-Catenin Rabbit polyclonal antibody (STJ27602) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101235/388727/STJ27602_4__62981.1713158266.jpg?c=1 "Immunohistochemistry analysis of Alpha-Catenin in paraffin-embedded human vermiform appendix using [KO Validated] Alpha-Catenin Rabbit polyclonal antibody (STJ27602) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of Alpha-Catenin in paraffin-embedded rat kidney using [KO Validated] Alpha-Catenin Rabbit polyclonal antibody (STJ27602) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101235/388728/STJ27602_5__23358.1713158267.jpg?c=1 "Immunohistochemistry analysis of Alpha-Catenin in paraffin-embedded rat kidney using [KO Validated] Alpha-Catenin Rabbit polyclonal antibody (STJ27602) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and Alpha-Catenin knockout (KO) 293T cells, using [KO Validated] Alpha-Catenin Rabbit polyclonal antibody (STJ27602) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101235/388729/STJ27602_6__37598.1713158268.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Alpha-Catenin knockout (KO) 293T cells, using [KO Validated] Alpha-Catenin Rabbit polyclonal antibody (STJ27602) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of various lysates using [KO Validated] Alpha-Catenin Rabbit polyclonal antibody (STJ27602) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101235/388730/STJ27602_7__42346.1713158268.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] Alpha-Catenin Rabbit polyclonal antibody (STJ27602) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
Anti-CTNNA1 antibody (637-906) (STJ27602)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Alpha-Catenin (637-906) antibody for use in WB, IHC-P, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CTNNA1 |
| Gene ID | 1495 |
| Uniprot ID | CTNA1_HUMAN |
| Immunogen Region | 637-906 |
| Immunogen Sequence | ELDDSDFETEDFDVRSRTSV QTEDDQLIAGQSARAIMAQL PQEQKAKIAEQVASFQEEKS KLDAEVSKWDDSGNDIIVLA KQMCMIMMEMTDFTRGKGPL KNTSDVISAAKKIAEAGSRM DKLGRTIADHCPDSACKQDL LAYLQRIALYCHQLNICSKV KAEVQNLGGELVVSGVDSAM SLIQAAKNLMNAVVQTVKAS YVASTKYQKSQGMASLNLPA VSWKMKAPEKKPLVKREKQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 637-906 of human Alpha-Catenin (NP_001894.2). |
Additional Info
| Tissue Specificity | Isoform 1: Ubiquitously expressed in normal tissues. Isoform 3: Abundantly expressed in brain and cerebellum, also expressed in the placenta, liver, lung, colon, heart, pancreas, stomach and thymus. |
| Post Translational Modifications | Sumoylated. Phosphorylation seems to contribute to the strength of cell-cell adhesion rather than to the basic capacity for cell-cell adhesion. |
| Function | Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. Involved in the regulation of WWTR1/TAZ, YAP1 and TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation. May play a crucial role in cell differentiation. |
| Protein Name | Catenin Alpha-1Alpha E-CateninCadherin-Associated ProteinRenal Carcinoma Antigen Ny-Ren-13 |
| Database Links | Reactome: R-HSA-418990Reactome: R-HSA-5218920Reactome: R-HSA-525793Reactome: R-HSA-5626467Reactome: R-HSA-9762292Reactome: R-HSA-9764302Reactome: R-HSA-9833576 |
| Cellular Localisation | CytoplasmCytoskeletonCell JunctionAdherens JunctionCell MembranePeripheral Membrane ProteinCytoplasmic SideNucleusFound At Cell-Cell Boundaries And Probably At Cell-Matrix BoundariesIsoform 3: Cell Membrane |
| Alternative Antibody Names | Anti-Catenin Alpha-1 antibodyAnti-Alpha E-Catenin antibodyAnti-Cadherin-Associated Protein antibodyAnti-Renal Carcinoma Antigen Ny-Ren-13 antibodyAnti-CTNNA1 antibody |
Information sourced from Uniprot.org