• Immunofluorescence analysis of HeLa cells using [KO Validated] DFFA Rabbit polyclonal antibody (STJ29835). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and DFFA knockout (KO) HeLa cells, using [KO Validated] DFFA Rabbit polyclonal antibody (STJ29835) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of various lysates using [KO Validated] DFFA Rabbit polyclonal antibody (STJ29835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-DFFA antibody (1-331) (STJ29835)

SKU:
STJ29835

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DFFA (1-331) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DFFA
Gene ID: 1676
Uniprot ID: DFFA_HUMAN
Immunogen Region: 1-331
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human DFFA (NP_004392.1).
Immunogen Sequence: MEVTGDAGVPESGEIRTLKP CLLRRNYSREQHGVAASCLE DLRSKACDILAIDKSLTPVT LVLAEDGTIVDDDDYFLCLP SNTKFVALASNEKWAYNNSD GGTAWISQESFDVDETDSGA GLKWKNVARQLKEDLSSIIL LSEEDLQMLVDAPCSDLAQE LRQSCATVQRLQHTLQQVLD QREEVRQSKQLLQLYLQALE KEGSLLSKQEESKAAFGEEV DAVDTGISRETSSDVALAS
Post Translational Modifications Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.
Function Inhibitor of the caspase-activated DNase (DFF40).
Protein Name Dna Fragmentation Factor Subunit Alpha
Dna Fragmentation Factor 45 Kda Subunit
Dff-45
Inhibitor Of Cad
Icad
Database Links Reactome: R-HSA-140342
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Dna Fragmentation Factor Subunit Alpha antibody
Anti-Dna Fragmentation Factor 45 Kda Subunit antibody
Anti-Dff-45 antibody
Anti-Inhibitor Of Cad antibody
Anti-Icad antibody
Anti-DFFA antibody
Anti-DFF1 antibody
Anti-DFF45 antibody
Anti-H13 antibody

Information sourced from Uniprot.org

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