![Immunofluorescence analysis of HeLa cells using [KO Validated] DFFA Rabbit polyclonal antibody (STJ29835). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/102903/392132/STJ29835_1__60621.1713162515.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KO Validated] DFFA Rabbit polyclonal antibody (STJ29835). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from wild type (WT) and DFFA knockout (KO) HeLa cells, using [KO Validated] DFFA Rabbit polyclonal antibody (STJ29835) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/102903/392133/STJ29835_2__21644.1713162517.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and DFFA knockout (KO) HeLa cells, using [KO Validated] DFFA Rabbit polyclonal antibody (STJ29835) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western blot analysis of various lysates using [KO Validated] DFFA Rabbit polyclonal antibody (STJ29835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/102903/392134/STJ29835_3__63133.1713162517.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] DFFA Rabbit polyclonal antibody (STJ29835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.")
![Immunofluorescence analysis of HeLa cells using [KO Validated] DFFA Rabbit polyclonal antibody (STJ29835). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/102903/392132/STJ29835_1__60621.1713162515.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KO Validated] DFFA Rabbit polyclonal antibody (STJ29835). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
Anti-DFFA antibody (1-331) (STJ29835)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DFFA (1-331) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DFFA |
| Gene ID | 1676 |
| Uniprot ID | DFFA_HUMAN |
| Immunogen Region | 1-331 |
| Immunogen Sequence | MEVTGDAGVPESGEIRTLKP CLLRRNYSREQHGVAASCLE DLRSKACDILAIDKSLTPVT LVLAEDGTIVDDDDYFLCLP SNTKFVALASNEKWAYNNSD GGTAWISQESFDVDETDSGA GLKWKNVARQLKEDLSSIIL LSEEDLQMLVDAPCSDLAQE LRQSCATVQRLQHTLQQVLD QREEVRQSKQLLQLYLQALE KEGSLLSKQEESKAAFGEEV DAVDTGISRETSSDVALAS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human DFFA (NP_004392.1). |
Additional Info
| Post Translational Modifications | Caspase-3 cleaves DFF45 at 2 sites to generate an active factor. |
| Function | Inhibitor of the caspase-activated DNase (DFF40). |
| Protein Name | Dna Fragmentation Factor Subunit AlphaDna Fragmentation Factor 45 Kda SubunitDff-45Inhibitor Of CadIcad |
| Database Links | Reactome: R-HSA-140342 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Dna Fragmentation Factor Subunit Alpha antibodyAnti-Dna Fragmentation Factor 45 Kda Subunit antibodyAnti-Dff-45 antibodyAnti-Inhibitor Of Cad antibodyAnti-Icad antibodyAnti-DFFA antibodyAnti-DFF1 antibodyAnti-DFF45 antibodyAnti-H13 antibody |
Information sourced from Uniprot.org