• Western blot analysis of extracts of various cell lines, using CIB1 antibody (STJ23139) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CIB1 antibody (1-191) (STJ23139)

SKU:
STJ23139

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CIB1 (1-191) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CIB1
Gene ID: 10519
Uniprot ID: CIB1_HUMAN
Immunogen Region: 1-191
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human CIB1 (NP_006375.2).
Immunogen Sequence: MGGSGSRLSKELLAEYQDLT FLTKQEILLAHRRFCELLPQ EQRSVESSLRAQVPFEQILS LPELKANPFKERICRVFSTS PAKDSLSFEDFLDLLSVFSD TATPDIKSHYAFRIFDFDDD GTLNREDLSRLVNCLTGEGE DTRLSASEMKQLIDNILEES DIDRDGTINLSEFQHVISRS PDFASSFKIVL
Tissue Specificity Ubiquitously expressed. Expressed in the epidermis, hair follicles and keratinocytes. Detected in platelets and in cell lines of megakaryocytic and erythrocytic lineages. Both isoform 1 and isoform 2 are detected in various cancer cell lines, with isoform 2 being the predominant form (at protein level).
Post Translational Modifications Phosphorylation of isoform 2 at Ser-118 by PRKD2 increases its ability to stimulate tumor angiogenesis.
Function Calcium-binding protein that plays a role in the regulation of numerous cellular processes, such as cell differentiation, cell division, cell proliferation, cell migration, thrombosis, angiogenesis, cardiac hypertrophy and apoptosis. Involved in bone marrow megakaryocyte differentiation by negatively regulating thrombopoietin-mediated signaling pathway. Participates in the endomitotic cell cycle of megakaryocyte, a form of mitosis in which both karyokinesis and cytokinesis are interrupted. Plays a role in integrin signaling by negatively regulating alpha-IIb/beta3 activation in thrombin-stimulated megakaryocytes preventing platelet aggregation. Up-regulates PTK2/FAK1 activity, and is also needed for the recruitment of PTK2/FAK1 to focal adhesions.it thus appears to play an important role in focal adhesion formation. Positively regulates cell migration on fibronectin in a CDC42-dependent manner, the effect being negatively regulated by PAK1. Functions as a negative regulator of stress activated MAP kinase (MAPK) signaling pathways. Down-regulates inositol 1,4,5-trisphosphate receptor-dependent calcium signaling. Involved in sphingosine kinase SPHK1 translocation to the plasma membrane in a N-myristoylation-dependent manner preventing TNF-alpha-induced apoptosis. Regulates serine/threonine-protein kinase PLK3 activity for proper completion of cell division progression. Plays a role in microtubule (MT) dynamics during neuronal development.disrupts the MT depolymerization activity of STMN2 attenuating NGF-induced neurite outgrowth and the MT reorganization at the edge of lamellipodia. Promotes cardiomyocyte hypertrophy via activation of the calcineurin/NFAT signaling pathway. Stimulates calcineurin PPP3R1 activity by mediating its anchoring to the sarcolemma. In ischemia-induced (pathological or adaptive) angiogenesis, stimulates endothelial cell proliferation, migration and microvessel formation by activating the PAK1 and ERK1/ERK2 signaling pathway. Promotes also cancer cell survival and proliferation. May regulate cell cycle and differentiation of spermatogenic germ cells, and/or differentiation of supporting Sertoli cells. Isoform 2: Plays a regulatory role in angiogenesis and tumor growth by mediating PKD/PRKD2-induced vascular endothelial growth factor A (VEGFA) secretion. (Microbial infection) Involved in keratinocyte-intrinsic immunity to human beta-papillomaviruses (HPVs).
Protein Name Calcium And Integrin-Binding Protein 1
Cib
Calcium- And Integrin-Binding Protein
Cibp
Calmyrin
Dna-Pkcs-Interacting Protein
Kinase-Interacting Protein
Kip
Snk-Interacting Protein 2-28
Sip2-28
Cellular Localisation Membrane
Lipid-Anchor
Cell Membrane
Sarcolemma
Apical Cell Membrane
Cell Projection
Ruffle Membrane
Filopodium Tip
Growth Cone
Lamellipodium
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Perinuclear Region
Nucleus
Neuron Projection
Perikaryon
Colocalized With Ppp3r1 At The Cell Membrane Of Cardiomyocytes In The Hypertrophic Heart
Colocalized With Nbr1 To The Perinuclear Region
Colocalizes With Tas1r2 In Apical Regions Of Taste Receptor Cells
Colocalized With Rac3 In The Perinuclear Area And At The Cell Periphery
Colocalized With Pak1 Within Membrane Ruffles During Cell Spreading Upon Readhesion To Fibronectin
Redistributed To The Cytoskeleton Upon Platelet Aggregation
Translocates From The Cytosol To The Plasma Membrane In A Calcium-Dependent Manner
Colocalized With Plk3 At Centrosomes In Ductal Breast Carcinoma Cells
Isoform 2: Cytoplasm
Golgi Apparatus
Trans-Golgi Network
Alternative Antibody Names Anti-Calcium And Integrin-Binding Protein 1 antibody
Anti-Cib antibody
Anti-Calcium- And Integrin-Binding Protein antibody
Anti-Cibp antibody
Anti-Calmyrin antibody
Anti-Dna-Pkcs-Interacting Protein antibody
Anti-Kinase-Interacting Protein antibody
Anti-Kip antibody
Anti-Snk-Interacting Protein 2-28 antibody
Anti-Sip2-28 antibody
Anti-CIB1 antibody
Anti-CIB antibody
Anti-KIP antibody
Anti-PRKDCIP antibody

Information sourced from Uniprot.org

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