Primary Antibodies | St John's Laboratory

Western blot analysis of various lysates using Phospho-I Kappa B Alpha-S36 Rabbit mAb (STJ11102588) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37 °C for overnight or treated by LPS (1 Mu g/mL) at 37 °C for 6 hours. Both NIH/3T3 cells and C6 cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-NFKBIA-S36 antibody [S8MR] (STJ11102588)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from C2C12 cells using Phospho-IRS1-S612 Rabbit pAb (STJ11102560) at 1:1000 dilution. C2C12 cells were treated by Insulin (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-IRS1-S612 antibody (STJ11102560)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using Nectin 2 Rabbit mAb (STJ11102509) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-NECTIN2 antibody (410-538) [S9MR] (STJ11102509)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from Rat liver, using Hemoglobin subunit alpha (HBA1) (HBA1) Rabbit mAb (STJ11102472) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-HBA1 antibody (1-100) [S2MR] (STJ11102472)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various lysates, using CD82 Rabbit mAb (STJ11102466) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-CD82 antibody (168-267) [S6MR] (STJ11102466)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of various lysates using GABA transaminase (GABA transaminase (ABAT) ) Rabbit mAb (STJ11102437) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-ABAT antibody (1-100) [S7MR] (STJ11102437)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using Alpha-Tubulin rabbit monoclonal antibody (STJ11102329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-TUBA4A antibody (349-448) [S9MR] (STJ11102329)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£169.50 - £314.50

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Confocal imaging of paraffin-embedded mouse large intestine using ADAM15 Rabbit monoclonal antibody (STJ11102325, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.

Anti-ADAM15 antibody (200-300) [S5MR] (STJ11102325)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using CHD3 rabbit monoclonal antibody (STJ11102316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CHD3 antibody (1-100) [S6MR] (STJ11102316)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Confocal imaging of paraffin-embedded Mouse colon tissue using ASH2L Rabbit monoclonal antibody (STJ11102184, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-ASH2L antibody (529-628) [S4MR] (STJ11102184)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of DKC1 in paraffin-embedded human spleen tissue using DKC1 Rabbit monoclonal antibody (STJ11102073) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-DKC1 antibody (300-400) [S3MR] (STJ11102073)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using UBE2I rabbit monoclonal antibody (STJ11102069) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-UBE2I antibody (1-100) [S9MR] (STJ11102069)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of mouse lung, using SMMHC/SMMHC/MYH11 rabbit monoclonal antibody (STJ11101988) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-MYH11 antibody (1160-1313) [S8MR] (STJ11101988)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of extracts of various cell lines, using USP7/HAUSP rabbit monoclonal antibody (STJ11101871) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-USP7 antibody (100-200) [S1MR] (STJ11101871)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using ACVR1B rabbit monoclonal antibody (STJ11101804) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-ACVR1B antibody (1-100) [S4MR] (STJ11101804)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g HuR/ELAVL1 antibody (STJ11101733). Western blot was performed from the immunoprecipitate using HuR/ELAVL1 antibody (STJ11101733) at a dilution of 1:1000.

Anti-ELAVL1 antibody (1-100) [S3MR] (STJ11101733)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-CDKN1A antibody (1-164) [S1MR] (STJ11101631)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of extracts of various cell lines, using GST3/GSTP1 antibody (STJ11101599) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-GSTP1 antibody (100-201) [S9MR] (STJ11101599)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-PCNA antibody (162-261) [S3MR] (STJ11101413)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/African green monkey

£143.50 - £618.50

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Western blot analysis of extracts from HeLa cells, using Na+/K+-ATPase rabbit monoclonal antibody (STJ11101388) at 1:50000 dilution. Membrane protein extract isolated from Hela cells. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ATP1A1 antibody (1-100) [S8MR] (STJ11101388)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of A-431 cells, using Cytokeratin 5 (KRT5) rabbit monoclonal antibody (STJ11101321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-KRT5 antibody (491-590) [S1MR] (STJ11101321)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using ATP5A1 rabbit monoclonal antibody (STJ11101295) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ATP5A1 antibody (200-300) [S5MR] (STJ11101295)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Western blot analysis of various lysates using Na+/K+-ATPase Rabbit pAb (STJ110188) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-ATP1A1 antibody (1-1023) (STJ110188)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using PCNA Mouse mAb (STJ11107563) at 1:5000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 10s.

Anti-PCNA antibody (1-261 aa) [SMM5496] (STJ11107563)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-MPO antibody (STJ13100488)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£322.50

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Anti-Spinophilin antibody (450-500 aa) (STJ13100376)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£322.50

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Anti-GFAP antibody (250-300) (STJ13100149)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

£322.50

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Anti-Clavesin 1 antibody (300-354 aa) (STJ13100108)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£322.50

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Anti-ATG16L1 antibody (STJ13100004)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£322.50

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Immunohistochemistry analysis of Transferrin. Sample: Human liver cancer, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG, Ready to use, RT, 20min.

Anti-Recombinant-Transferrin antibody [SR0697] (STJA0010697)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human

£153.50 - £228.50

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Anti-Recombinant-Annexin-6/ANXA6 antibody [SM0597] (STJA0010597)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Anti-Recombinant-Glutamine Synthetase antibody [SM0584] (STJA0010584)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Anti-Recombinant-Heme Oxygenase 1 antibody [SM0572] (STJA0010572)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Immunohistochemistry analysis of Granzyme B. Sample: Rat thymus, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:800, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG, Ready to use, RT, 20min.

Anti-Recombinant-Phospho-Granzyme B antibody [SR0537] (STJA0010537)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Rat

£153.50 - £228.50

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Anti-Caldesmon/CDM antibody [SM0510] (STJA0010510)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Anti-METTL3 antibody [SM0492] (STJA0010492)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Immunohistochemistry analysis of Ly6g. Sample: mouse Inflammatory model induced by lps liver, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rat lgG, Ready to use, RT, 20min.

Anti-Ly6g antibody [SM0468] (STJA0010468)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Mouse

£153.50 - £228.50

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Western blot analysis of Myelin Basic Protein at dilution of 1:2000

Anti-Myelin Basic Protein antibody [SM0466] (STJA0010466)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Anti-Collagen III antibody [SM0439] (STJA0010439)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£153.50 - £228.50

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Anti-NAPSIN A antibody [SM0409] (STJA0010409)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Anti-CD11b antibody [SM0390] (STJA0010390)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Immunohistochemistry analysis of PMS2. Sample: Human colon, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Primary antibody: 1:3000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200, RT, 1h.

Anti-PMS2 antibody [SM0372] (STJA0010372)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Immunohistochemistry of paraffin embedded human gastric cancer using Na K-ATpase at dilution of 1:1000

Anti-Sodium Potassium ATPase antibody [SM5233] (STJA0005233)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Western blot analysis of a-Tubulin at dilution of 1:2000

Anti-alpha Tubulin antibody [SM5232] (STJA0005232)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Immunohistochemistry analysis of CD34. Sample: Human breast cancer, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.

Anti-CD34 antibody [SM5231] (STJA0005231)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Western blot analysis of PGP9. 5. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:3000, RT, 1h.

Anti-PGP9.5 antibody [SM5228] (STJA0005228)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Western blot analysis of Cyt-C at dilution of 1:1000

Anti-Cytochrome C antibody [SM5173] (STJA0005173)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

£153.50 - £228.50

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Anti-COX IV antibody [SM5083] (STJA0005083)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ICC

Reactivity :

Mouse/Rat

£153.50 - £228.50

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