• Western blot analysis of extracts of various cell lines, using Alpha-Tubulin rabbit monoclonal antibody (STJ11102329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunohistochemistry analysis of paraffin-embedded human colon using Alpha-Tubulin rabbit monoclonal antibody (STJ11102329) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using Alpha-Tubulin rabbit monoclonal antibody (STJ11102329) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using Alpha-Tubulin rabbit monoclonal antibody (STJ11102329) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using Alpha-Tubulin rabbit monoclonal antibody (STJ11102329) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using Alpha-Tubulin rabbit monoclonal antibody (STJ11102329) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using Alpha-Tubulin rabbit monoclonal antibody (STJ11102329) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300 Mu g extracts from HeLa cells using 3 Mu g Alpha-Tubulin rabbit monoclonal antibody (STJ11102329). Western blot was performed from the immunoprecipitate using Alpha-Tubulin rabbit monoclonal antibody (STJ11102329) at a dilution of 1:1000.
  • Anti-TUBA4A antibody (349-448) [S9MR] (STJ11102329)

Anti-TUBA4A antibody (349-448) [S9MR] (STJ11102329)

SKU:
STJ11102329

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Alpha-Tubulin (349-448) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TUBA4A
Gene ID: 7277
Uniprot ID: TBA4A_HUMAN
Immunogen Region: 349-448
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 349-448 of human Alpha-Tubulin (P68366).
Immunogen Sequence: TGFKVGINYQPPTVVPGGDL AKVQRAVCMLSNTTAIAEAW ARLDHKFDLMYAKRAFVHWY VGEGMEEGEFSEAREDMAAL EKDYEEVGIDSYEDEDEGEE
Post Translational Modifications Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Glutamylation is also involved in cilia motility. Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility. Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly. Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability. Although this tubulin does not encode a C-terminal tyrosine, a C-terminal tyrosine can be added post-translationally by the tubulin tyrosine ligase (TTL). It can then undergo a detyrosination cycle by the tubulin tyrosine carboxypeptidase (KIAA0895L/MATCAP).
Function Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Protein Name Tubulin Alpha-4a Chain
Alpha-Tubulin 1
Testis-Specific Alpha-Tubulin
Tubulin H2-Alpha
Tubulin Alpha-1 Chain
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-1445148
Reactome: R-HSA-190840
Reactome: R-HSA-190861
Reactome: R-HSA-2132295
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-2565942
Reactome: R-HSA-3371497
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-389957
Reactome: R-HSA-389960
Reactome: R-HSA-389977
Reactome: R-HSA-437239
Reactome: R-HSA-5610787
Reactome: R-HSA-5617833
Reactome: R-HSA-5620912
Reactome: R-HSA-5620924
Reactome: R-HSA-5626467
Reactome: R-HSA-5663220
Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Reactome: R-HSA-6811436
Reactome: R-HSA-68877
Reactome: R-HSA-8852276
Reactome: R-HSA-8854518
Reactome: R-HSA-8955332
Reactome: R-HSA-9609690
Reactome: R-HSA-9609736
Reactome: R-HSA-9619483
Reactome: R-HSA-9646399
Reactome: R-HSA-9648025
Reactome: R-HSA-9668328
Reactome: R-HSA-983189
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Tubulin Alpha-4a Chain antibody
Anti-Alpha-Tubulin 1 antibody
Anti-Testis-Specific Alpha-Tubulin antibody
Anti-Tubulin H2-Alpha antibody
Anti-Tubulin Alpha-1 Chain antibody
Anti-TUBA4A antibody
Anti-TUBA1 antibody

Information sourced from Uniprot.org

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