• Western blot analysis of extracts of various cell lines, using ATP5A1 rabbit monoclonal antibody (STJ11101295) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry of paraffin-embedded rat kidney using ATP5A1 rabbit monoclonal antibody (STJ11101295) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using ATP5A1 rabbit monoclonal antibody (STJ11101295) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse spleen using ATP5A1 rabbit monoclonal antibody (STJ11101295) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using ATP5A1 rabbit monoclonal antibody (STJ11101295) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using ATP5A1 rabbit monoclonal antibody (STJ11101295) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATP5A1 antibody (200-300) [S5MR] (STJ11101295)

SKU:
STJ11101295

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ATP5A1 (200-300) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP5F1A
Gene ID: 498
Uniprot ID: ATPA_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ATP5A1 (P25705).
Immunogen Sequence: VPIGRGQRELIIGDRQTGKT SIAIDTIINQKRFNDGSDEK KKLYCIYVAIGQKRSTVAQL VKRLTDADAMKYTIVVSATA SDAAPLQYLAPYSGCSMGEY F
Tissue Specificity Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.
Post Translational Modifications The N-terminus is blocked. Acetylated on lysine residues. BLOC1S1 is required for acetylation.
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites. Binds the bacterial siderophore enterobactin and can promote mitochondrial accumulation of enterobactin-derived iron ions.
Protein Name Atp Synthase Subunit Alpha - Mitochondrial
Atp Synthase F1 Subunit Alpha
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-163210
Reactome: R-HSA-8949613
Cellular Localisation Mitochondrion
Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Cell Membrane
Extracellular Side
Colocalizes With Hrg On The Cell Surface Of T-Cells
Alternative Antibody Names Anti-Atp Synthase Subunit Alpha - Mitochondrial antibody
Anti-Atp Synthase F1 Subunit Alpha antibody
Anti-ATP5F1A antibody
Anti-ATP5A antibody
Anti-ATP5A1 antibody
Anti-ATP5AL2 antibody
Anti-ATPM antibody

Information sourced from Uniprot.org

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