• Western blot analysis of extracts of Rat liver, using Hemoglobin subunit alpha (HBA1) (HBA1) rabbit monoclonal antibody (STJ11102472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded red blood cell staining in rat kidney using Hemoglobin subunit alpha (HBA1) (HBA1) rabbit monoclonal antibody (STJ11102472) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded blood cell staining in human placenta using Hemoglobin subunit alpha (HBA1) (HBA1) rabbit monoclonal antibody (STJ11102472) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded blood cell staining in mouse lung using Hemoglobin subunit alpha (HBA1) (HBA1) rabbit monoclonal antibody (STJ11102472) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using Hemoglobin subunit alpha (HBA1) rabbit monoclonal antibody (STJ11102472) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human kidney using Hemoglobin subunit alpha (HBA1) rabbit monoclonal antibody (STJ11102472) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using Hemoglobin subunit alpha (HBA1) rabbit monoclonal antibody (STJ11102472) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse liver using Hemoglobin subunit alpha (HBA1) rabbit monoclonal antibody (STJ11102472) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using Hemoglobin subunit alpha (HBA1) rabbit monoclonal antibody (STJ11102472) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of rat spleen using Hemoglobin subunit alpha (HBA1) (HBA1) rabbit monoclonal antibody (STJ11102472) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of human spleen using Hemoglobin subunit alpha (HBA1) (HBA1) rabbit monoclonal antibody (STJ11102472) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse spleen using Hemoglobin subunit alpha (HBA1) (HBA1) rabbit monoclonal antibody (STJ11102472) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HBA1 antibody (1-100) [S2MR] (STJ11102472)

SKU:
STJ11102472

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Hemoglobin subunit alpha (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HBA1.HBA2
Gene ID: 3039/3040
Uniprot ID: HBA_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Hemoglobin subunit alpha (HBA1) (HBA1) (P69905).
Immunogen Sequence: MVLSPADKTNVKAAWGKVGA HAGEYGAEALERMFLSFPTT KTYFPHFDLSHGSAQVKGHG KKVADALTNAVAHVDDMPNA LSALSDLHAHKLRVDPVNFK
Tissue Specificity Red blood cells.
Post Translational Modifications The initiator Met is not cleaved in variant Thionville and is acetylated.
Function Involved in oxygen transport from the lung to the various peripheral tissues. Hemopressin: Hemopressin acts as an antagonist peptide of the cannabinoid receptor CNR1. Hemopressin-binding efficiently blocks cannabinoid receptor CNR1 and subsequent signaling.
Protein Name Hemoglobin Subunit Alpha
Alpha-Globin
Hemoglobin Alpha Chain Cleaved Into - Hemopressin
Database Links Reactome: R-HSA-1237044
Reactome: R-HSA-1247673
Reactome: R-HSA-2168880
Reactome: R-HSA-9707564
Reactome: R-HSA-9707616
Alternative Antibody Names Anti-Hemoglobin Subunit Alpha antibody
Anti-Alpha-Globin antibody
Anti-Hemoglobin Alpha Chain Cleaved Into - Hemopressin antibody
Anti-HBA1.HBA2 antibody

Information sourced from Uniprot.org

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