• Western blot analysis of extracts of various cell lines, using GABA transaminase (GABA transaminase (ABAT) ) rabbit monoclonal antibody (STJ11102437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of ABAT in paraffin-embedded human liver cancer tissue using GABA transaminase (ABAT) Rabbit monoclonal antibody (STJ11102437) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded rat brain using GABA transaminase (GABA transaminase (ABAT) ) rabbit monoclonal antibody (STJ11102437) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of ABAT in paraffin-embedded human liver tissue using GABA transaminase (ABAT) Rabbit monoclonal antibody (STJ11102437) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded human liver cancer using GABA transaminase (GABA transaminase (ABAT) ) rabbit monoclonal antibody (STJ11102437) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of GABA transaminase (ABAT) in paraffin-embedded mouse spinal cord using GABA transaminase (GABA transaminase (ABAT) ) Rabbit monoclonal antibody (STJ11102437) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse spinal cord using GABA transaminase (GABA transaminase (ABAT) ) rabbit monoclonal antibody (STJ11102437) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of GABA transaminase (ABAT) in paraffin-embedded rat brain using GABA transaminase (GABA transaminase (ABAT) ) Rabbit monoclonal antibody (STJ11102437) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using GABA transaminase (GABA transaminase (ABAT) ) Rabbit monoclonal antibody (STJ11102437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ABAT antibody (1-100) [S7MR] (STJ11102437)

SKU:
STJ11102437

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ABAT (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ABAT
Gene ID: 18
Uniprot ID: GABT_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GABA transaminase (ABAT) (P80404).
Immunogen Sequence: MASMLLAQRLACSFQHSYRL LVPGSRHISQAAAKVDVEFD YDGPLMKTEVPGPRSQELMK QLNIIQNAEAVHFFCNYEES RGNYLVDVDGNRMLDLYSQI
Tissue Specificity Liver > pancreas > brain > kidney > heart > placenta.
Function Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.
Protein Name 4-Aminobutyrate Aminotransferase - Mitochondrial
(S-3-Amino-2-Methylpropionate Transaminase
Gaba Aminotransferase
Gaba-At
Gamma-Amino-N-Butyrate Transaminase
Gaba Transaminase
Gaba-T
L-Aibat
Database Links Reactome: R-HSA-916853
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-4-Aminobutyrate Aminotransferase - Mitochondrial antibody
Anti-(S-3-Amino-2-Methylpropionate Transaminase antibody
Anti-Gaba Aminotransferase antibody
Anti-Gaba-At antibody
Anti-Gamma-Amino-N-Butyrate Transaminase antibody
Anti-Gaba Transaminase antibody
Anti-Gaba-T antibody
Anti-L-Aibat antibody
Anti-ABAT antibody
Anti-GABAT antibody

Information sourced from Uniprot.org

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