• Western blot analysis of extracts of mouse lung, using SMMHC/SMMHC/MYH11 rabbit monoclonal antibody (STJ11101988) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts of Rat lung, using SMMHC/SMMHC/MYH11 rabbit monoclonal antibody (STJ11101988) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using SMMHC/MYH11 rabbit monoclonal antibody (STJ11101988) at dilution of 1:8000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using SMMHC/MYH11 rabbit monoclonal antibody (STJ11101988) at dilution of 1:8000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using SMMHC/MYH11 rabbit monoclonal antibody (STJ11101988) at dilution of 1:8000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using SMMHC/MYH11 rabbit monoclonal antibody (STJ11101988) at dilution of 1:8000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of human colon using SMMHC/MYH11 rabbit monoclonal antibody (STJ11101988) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse colon using SMMHC/MYH11 rabbit monoclonal antibody (STJ11101988) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat colon using SMMHC/MYH11 rabbit monoclonal antibody (STJ11101988) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-MYH11 antibody (1160-1313) [S8MR] (STJ11101988)

SKU:
STJ11101988

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MYH11 (1160-1313) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:2000-1:10000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYH11
Gene ID: 4629
Uniprot ID: MYH11_HUMAN
Immunogen Region: 1160-1313
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1160-1313 of human SMMHC/MYH11 (NP_002465.1).
Immunogen Sequence: DSTATQQELRAKREQEVTVL KKALDEETRSHEAQVQEMRQ KHAQAVEELTEQLEQFKRAK ANLDKNKQTLEKENADLAGE LRVLGQAKQEVEHKKKKLEA QVQELQSKCSDGERARAELN DKVHKLQNEVESVTGMLNEA EGKAIKLAKDVASL
Tissue Specificity Smooth muscle.expressed in the umbilical artery, bladder, esophagus and trachea. Isoform 1 is mostly found in slowly contracting tonic muscles.
Function Muscle contraction.
Protein Name Myosin-11
Myosin Heavy Chain 11
Myosin Heavy Chain - Smooth Muscle Isoform
Smmhc
Database Links Reactome: R-HSA-3928663
Reactome: R-HSA-416572
Reactome: R-HSA-445355
Reactome: R-HSA-5625740
Reactome: R-HSA-5625900
Reactome: R-HSA-5627117
Reactome: R-HSA-5627123
Cellular Localisation Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Thick Filaments Of The Myofibrils
Alternative Antibody Names Anti-Myosin-11 antibody
Anti-Myosin Heavy Chain 11 antibody
Anti-Myosin Heavy Chain - Smooth Muscle Isoform antibody
Anti-Smmhc antibody
Anti-MYH11 antibody
Anti-KIAA0866 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance