• Western blot analysis of extracts of A-431 cells, using Cytokeratin 5 (KRT5) rabbit monoclonal antibody (STJ11101321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of extracts of various cell lines, using Cytokeratin 5 (KRT5) rabbit monoclonal antibody (STJ11101321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Cytokeratin 5 (KRT5) rabbit monoclonal antibody (STJ11101321) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using Cytokeratin 5 (KRT5) rabbit monoclonal antibody (STJ11101321) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using Cytokeratin 5 (KRT5) rabbit monoclonal antibody (STJ11101321) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung squamous carcinoma tissue using Cytokeratin 5 (KRT5) rabbit monoclonal antibody (STJ11101321) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Confocal imaging of paraffin-embedded Human skin using Cytokeratin 5 (CK5) rabbit monoclonal antibody (STJ11101321, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded mouse skin using Cytokeratin 5 (CK5) rabbit monoclonal antibody (STJ11101321, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Rat skin using Cytokeratin 5 (CK5) rabbit monoclonal antibody (STJ11101321, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.

Anti-KRT5 antibody (491-590) [S1MR] (STJ11101321)

SKU:
STJ11101321

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Cytokeratin 5 (491-590) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT5
Gene ID: 3852
Uniprot ID: K2C5_HUMAN
Immunogen Region: 491-590
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 491-590 of human Cytokeratin 5 (KRT5) (P13647).
Immunogen Sequence: ISVVTSSVSSGYGSGSGYGG GLGGGLGGGLGGGLAGGSSG SYYSSSSGGVGLGGGLSVGG SGFSASSGRGLGVGFGSGGG SSSSVKFVSTTSSSRKSFKS
Tissue Specificity Expressed in corneal epithelium (at protein level). Expressed in keratinocytes (at protein level).
Post Translational Modifications Phosphorylated by CDK1, AURKB and Rho-kinase, phosphorylation is regulated by the cell cycle. Thr-24 phosphorylation, mediated by CDK1, peaks during prometaphase or metaphase cells with phosphorylated filamentous structures evident throughout the cytoplasm during early mitosis. CDK1 phosphorylates Thr-24 in mitotic cells at the site of injury. O-glycosylated.
Function Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress. Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes.
Protein Name Keratin - Type Ii Cytoskeletal 5
58 Kda Cytokeratin
Cytokeratin-5
Ck-5
Keratin-5
K5
Type-Ii Keratin Kb5
Database Links Reactome: R-HSA-446107
Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Keratin - Type Ii Cytoskeletal 5 antibody
Anti-58 Kda Cytokeratin antibody
Anti-Cytokeratin-5 antibody
Anti-Ck-5 antibody
Anti-Keratin-5 antibody
Anti-K5 antibody
Anti-Type-Ii Keratin Kb5 antibody
Anti-KRT5 antibody

Information sourced from Uniprot.org

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