Anti-KRT5 antibody (491-590) [S1MR] (STJ11101321)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Cytokeratin 5 (491-590) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S1MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000IHC-P:1:400-1:4000IF/ICC:1:200-1:400ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KRT5 |
| Gene ID | 3852 |
| Uniprot ID | K2C5_HUMAN |
| Immunogen Region | 491-590 |
| Immunogen Sequence | ISVVTSSVSSGYGSGSGYGG GLGGGLGGGLGGGLAGGSSG SYYSSSSGGVGLGGGLSVGG SGFSASSGRGLGVGFGSGGG SSSSVKFVSTTSSSRKSFKS |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 491-590 of human Cytokeratin 5 (KRT5) (P13647). |
Additional Info
| Tissue Specificity | Expressed in corneal epithelium (at protein level). Expressed in keratinocytes (at protein level). |
| Post Translational Modifications | Phosphorylated by CDK1, AURKB and Rho-kinase, phosphorylation is regulated by the cell cycle. Thr-24 phosphorylation, mediated by CDK1, peaks during prometaphase or metaphase cells with phosphorylated filamentous structures evident throughout the cytoplasm during early mitosis. CDK1 phosphorylates Thr-24 in mitotic cells at the site of injury. O-glycosylated. |
| Function | Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress. Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes. |
| Protein Name | Keratin - Type Ii Cytoskeletal 558 Kda CytokeratinCytokeratin-5Ck-5Keratin-5K5Type-Ii Keratin Kb5 |
| Database Links | Reactome: R-HSA-446107Reactome: R-HSA-6805567Reactome: R-HSA-6809371Reactome: R-HSA-9725554 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Keratin - Type Ii Cytoskeletal 5 antibodyAnti-58 Kda Cytokeratin antibodyAnti-Cytokeratin-5 antibodyAnti-Ck-5 antibodyAnti-Keratin-5 antibodyAnti-K5 antibodyAnti-Type-Ii Keratin Kb5 antibodyAnti-KRT5 antibody |
Information sourced from Uniprot.org