• Western blot analysis of extracts of various cell lines, using Nectin 2 rabbit monoclonal antibody (STJ11102509) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of Nectin 2/CD112 in paraffin-embedded human thyroid cancer tissue using Nectin 2/CD112 Rabbit monoclonal antibody (STJ11102509) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded rat testis using Nectin 2/CD112 rabbit monoclonal antibody (STJ11102509) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Nectin 2/CD112 in paraffin-embedded mouse testis tissue using Nectin 2/CD112 Rabbit monoclonal antibody (STJ11102509) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded human placenta using Nectin 2/CD112 rabbit monoclonal antibody (STJ11102509) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Nectin 2/CD112 in paraffin-embedded human colon tissue using Nectin 2/CD112 Rabbit monoclonal antibody (STJ11102509) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded mouse kidney using Nectin 2/CD112 rabbit monoclonal antibody (STJ11102509) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Nectin 2/CD112 in paraffin-embedded rat colon tissue using Nectin 2/CD112 Rabbit monoclonal antibody (STJ11102509) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using Nectin 2 Rabbit monoclonal antibody (STJ11102509) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NECTIN2 antibody (410-538) [S9MR] (STJ11102509)

SKU:
STJ11102509

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Nectin 2/CD112 (410-538) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NECTIN2
Gene ID: 5819
Uniprot ID: NECT2_HUMAN
Immunogen Region: 410-538
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 410-538 of human Nectin 2/CD112 (ARC1665).
Immunogen Sequence: TPKAKLEAQEMPSQLFTLGA SEHSPLKTPYFDAGASCTEQ EMPRYHELPTLEERSGPLHP GATSLGSPIPVPPGPPAVED VSLDLEDEEGEEEEEYLDKI NPIYDALSYSSPSDSYQGKG FVMSRAMYV
Tissue Specificity Ubiquitous.
Function Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive. Probable cell adhesion protein. (Microbial infection) Acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV).
Protein Name Nectin-2
Herpes Virus Entry Mediator B
Herpesvirus Entry Mediator B
Hveb
Nectin Cell Adhesion Molecule 2
Poliovirus Receptor-Related Protein 2
Cd Antigen Cd112
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-418990
Reactome: R-HSA-420597
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Nectin-2 antibody
Anti-Herpes Virus Entry Mediator B antibody
Anti-Herpesvirus Entry Mediator B antibody
Anti-Hveb antibody
Anti-Nectin Cell Adhesion Molecule 2 antibody
Anti-Poliovirus Receptor-Related Protein 2 antibody
Anti-Cd Antigen Cd112 antibody
Anti-NECTIN2 antibody
Anti-HVEB antibody
Anti-PRR2 antibody
Anti-PVRL2 antibody

Information sourced from Uniprot.org

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