Muscle Contraction | Research Area | St John's Laboratory

Western blot analysis of lysates from Rat skeletal muscle, using Troponin I1/TnI1 Rabbit mAb (STJ11103673) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Troponin I1/TnI1 antibody (1-187) [S3MR] (STJ11103673)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$174.83 - $775.58

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Western blot analysis of various lysates using TRPC1 Rabbit pAb (STJ111993) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-TRPC1 antibody (400-500) (STJ111993)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using TRPC1 antibody (STJ114399) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TRPC1 antibody (400-500) (STJ114399)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using TRPC1 Rabbit monoclonal antibody (STJ11101983) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-TRPC1 antibody (400-500) [S3MR] (STJ11101983)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Anti-TRPC1 antibody (STJ191415)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded human-heart, antibody was diluted at 1:200

Anti-TTN antibody (161-210 aa) (STJ98821)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of paraffin-embedded mouse heart using TTN Rabbit polyclonal antibody (STJ119141) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TTN antibody (5347-5604) (STJ119141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse/Rat

$151.88 - $708.08

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Anti-VCL antibody (100-200) [PT0372] (STJA0006223)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of NIH/3T3 cells using Vinculin antibody (STJ116126) at dilution of 1:150. Blue: DAPI for nuclear staining.

Anti-VCL antibody (751-850) (STJ116126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using Vinculin antibody (STJ26077) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-VCL antibody (767-1066) (STJ26077)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various cells using Vinculin Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Anti-VCL antibody (786-835 aa) (STJ96247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Confocal imaging of HeLa cells using Vinculin Rabbit monoclonal antibody (STJ11101832, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-VCL antibody (800-900) [S2MR] (STJ11101832)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of Mouse brain, using Vinculin antibody (STJ11104807) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-VCL antibody (800-900) [S4807RM] (STJ11104807)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Western blot analysis of various lysates using Vimentin Rabbit pAb (STJ26092) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-VIM antibody (1-200) (STJ26092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of U2OS cells using [KD Validated] Vimentin Rabbit polyclonal antibody (STJ113868) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-VIM antibody (1-466) (STJ113868)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Immunofluorescence analysis of U-2 OS cells using Vimentin Rabbit polyclonal antibody (STJ11104460) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-VIM antibody (10-101) (STJ11104460)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemistry analysis of Vimentin in paraffin-embedded rat spleen using Vimentin Rabbit polyclonal antibody (STJ113129) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-VIM antibody (300-400) (STJ113129)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-VIM antibody (367-466) [S7MR] (STJ11101727)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Anti-VIM antibody at 1:2500 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-VIM antibody (390aa C-Term) (STJ140133)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey/Canine

$182.93 - $532.58

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Human appendix tissue was stained with Anti-Vimentin (ABT019) Antibody

Anti-VIM antibody (400-466 aa) [ABT019] (STJ197249)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$234.90 - $340.20

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U2OS whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Vimentin (ABT281) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: U2OSPredicted band size: 54kDaObserved band size: 54kDa

Anti-VIM antibody (400-466 aa) [ABT281] (STJ197198)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

$234.90 - $340.20

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Immunocytochemistry analysis of Vimentin (green) in Hela using Vimentin antibody, and DAPI (blue).

Anti-VIM antibody (448-465 aa) [R02-8C4] (STJA0013017)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat/Hamster

$357.08

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Immunofluorescence analysis of Hela cell. 1, Vimentin Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-VIM antibody (56-105 aa) (STJ96245)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain tissue, diluted at 1:2000.

Anti-VIM antibody [1A7] (STJ97060)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot detection of Vimentin in Molt-4, K562, COS7, Jurkat, Hela and Vimentin negative cell (Daudi, Ramos, Raji) lysates using Vimentin mouse mAb (1:1000 diluted).Predicted band size:57KDa.Observed band size:57KDa.

Anti-VIM antibody [4F8-A5-H5] (STJ99270)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human

$194.40 - $469.80

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Anti-VIM/Vimentin antibody [SAA0112] (STJA0026948)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$343.58 - $510.98

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Anti-VIM/Vimentin antibody [SAA0112] {APC} (STJA0029094)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$419.18 - $637.88

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Anti-VIM/Vimentin antibody [SAA0112] {FITC} (STJA0028128)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$343.58 - $510.98

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Anti-VIM/Vimentin antibody [SAA0112] {PerCP} (STJA0030060)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$394.88 - $594.68

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Anti-VIM/Vimentin antibody [SAA0112] {PE} (STJA0031026)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$419.18 - $637.88

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Anti-VIM/Vimentin antibody [VB6] (STJA0026947)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human

$343.58 - $510.98

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Anti-VIM/Vimentin antibody [VB6] {APC} (STJA0029093)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human

$419.18 - $637.88

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Anti-VIM/Vimentin antibody [VB6] {FITC} (STJA0028127)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human

$343.58 - $510.98

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Anti-VIM/Vimentin antibody [VB6] {PerCP} (STJA0030059)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human

$394.88 - $594.68

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Anti-VIM/Vimentin antibody [VB6] {PE} (STJA0031025)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human

$419.18 - $637.88

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Anti-VIM/Vimentin nanobody [SAA1226] (STJN000124)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/IF/SPR/WB

Reactivity :

Human

$510.98 - $2,203.88

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Formalin-fixed, paraffin-embedded human gastrointestinal stromal tumor stained with anti-vimentin antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells

Anti-Vimentin antibody (2-466aa) [ZR381] (STJ180548)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$218.03 - $670.28

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Immunostaining of tumors and precursor PanIN lesions from 4 week old LSL-KrasG12D; Cdkn2alox/lox; p48Cre (KIC) mice that were treated with 2G8 and/or gemcitabine, Gem. (Anti-Vimentin, cat. STJA0003823, green; Anti-E-cadherin, red). Photo courtesy of Rolf Brekken, University of Texas Southwestern. CC-BY-4. 0

Anti-Vimentin antibody (STJA0003823)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Feline/Goat/Guinea Pig/Hamster/Horse/Non-Human Primates/Rabbit/Sheep/Vole

$544.73

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Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa vimentin protein.

Anti-Vimentin antibody [2D1] (STJA0003824)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/D.melanogaster/Feline/Finch/Fish/Goat/Guinea Pig/Hamster/Horse/Lizard/Non-Human Primates/Rabbit/Sheep/Vole/Xenopus/Zebrafish

$613.58

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Anti-Vimentin antibody [65E] (STJ16100819)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/WB

Reactivity :

Human

$818.78

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Anti-Vimentin antibody [EBS-O-184] (STJ16102604)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Human

$415.13 - $1,956.83

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Anti-Vimentin antibody [LN-6] (STJ16101027)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Cat/Cow/Human/Mouse/Pig/Rabbit/Rat/Sheep

$415.13 - $1,956.83

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Anti-Vimentin antibody [LN6] (STJA0003872)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human

$613.58

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Anti-Vimentin antibody [SM0430] (STJA0010430)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

$230.18 - $315.23

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Anti-Vimentin antibody [SM5190] (STJA0005190)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

$315.23

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Anti-Vimentin antibody [SRL33] (STJ16101801)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$1,010.48

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Anti-Vimentin antibody [V9] (STJ16100234)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/IHC-F/WB/FC

Reactivity :

Human

$369.23

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Anti-Vimentin antibody [V9] (STJ16101600)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$766.13

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