Anti-RYR2 antibody (1350-1400 aa) (STJ13101950)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from aa region 1350-1400 of human Ryanodine receptor 2 (RYR2) conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
General Information
| Short Description | Rabbit polyclonal anti-RYR2 (1350-1400 aa) for use in IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB (confirmed by recombinant protein). A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | RYR2 |
| Gene ID | 6262 |
| Uniprot ID | RYR2_HUMAN |
| Immunogen | A synthetic peptide from aa region 1350-1400 of human Ryanodine receptor 2 (RYR2) conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse. |
| Immunogen Region | 1350-1400 aa |
| Specificity | Specific for RYR2. |
Additional Info
| Post Translational Modifications | Channel activity is modulated by phosphorylation. Phosphorylation at Ser-2808 and Ser-2814 increases the open probability of the calcium channel. Phosphorylation is increased in failing heart, leading to calcium leaks and increased cytoplasmic Ca(2+) levels. Phosphorylation at Ser-2031 by PKA enhances the response to lumenal calcium. |
| Function | Cytosolic calcium-activated calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytosol and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) cytosolic levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development. |
| Protein Name | Ryanodine Receptor 2Ryr-2Ryr2Hryr-2Cardiac Muscle Ryanodine ReceptorCardiac Muscle Ryanodine Receptor-Calcium Release ChannelType 2 Ryanodine Receptor |
| Database Links | Reactome: R-HSA-2672351Reactome: R-HSA-5578775 |
| Cellular Localisation | Sarcoplasmic Reticulum MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Ryanodine Receptor 2 antibodyAnti-Ryr-2 antibodyAnti-Ryr2 antibodyAnti-Hryr-2 antibodyAnti-Cardiac Muscle Ryanodine Receptor antibodyAnti-Cardiac Muscle Ryanodine Receptor-Calcium Release Channel antibodyAnti-Type 2 Ryanodine Receptor antibodyAnti-RYR2 antibody |
Information sourced from Uniprot.org