Anti-RYR1 antibody (1360-1400 aa) (STJ13100310)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from aa region 1360-1400 of human RYR1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
General Information
| Short Description | Rabbit polyclonal anti-RYR1 (1360-1400 aa) for use in IHC and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB (confirmed by recombinant protein). A dilution of 1:1000 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | RYR1 |
| Gene ID | 6261 |
| Uniprot ID | RYR1_HUMAN |
| Immunogen | A synthetic peptide from aa region 1360-1400 of human RYR1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse. |
| Immunogen Region | 1360-1400 aa |
| Specificity | Specific for RYR1. |
Additional Info
| Post Translational Modifications | Channel activity is modulated by phosphorylation. Phosphorylation at Ser-2843 may increase channel activity. Repeated very high-level exercise increases phosphorylation at Ser-2843. Activated by reversible S-nitrosylation. Repeated very high-level exercise increases S-nitrosylation. |
| Function | Cytosolic calcium-activated calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytosol and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules. Repeated very high-level exercise increases the open probability of the channel and leads to Ca(2+) leaking into the cytoplasm. Can also mediate the release of Ca(2+) from intracellular stores in neurons, and may thereby promote prolonged Ca(2+) signaling in the brain. Required for normal embryonic development of muscle fibers and skeletal muscle. Required for normal heart morphogenesis, skin development and ossification during embryogenesis. |
| Protein Name | Ryanodine Receptor 1Ryr-1Ryr1Skeletal Muscle Calcium Release ChannelSkeletal Muscle Ryanodine ReceptorSkeletal Muscle-Type Ryanodine ReceptorType 1 Ryanodine Receptor |
| Database Links | Reactome: R-HSA-2672351Reactome: R-HSA-5578775 |
| Cellular Localisation | Sarcoplasmic Reticulum MembraneMulti-Pass Membrane ProteinThe Number Of Predicted Transmembrane Domains Varies Between OrthologsBoth N-Terminus And C-Terminus Are Cytoplasmic |
| Alternative Antibody Names | Anti-Ryanodine Receptor 1 antibodyAnti-Ryr-1 antibodyAnti-Ryr1 antibodyAnti-Skeletal Muscle Calcium Release Channel antibodyAnti-Skeletal Muscle Ryanodine Receptor antibodyAnti-Skeletal Muscle-Type Ryanodine Receptor antibodyAnti-Type 1 Ryanodine Receptor antibodyAnti-RYR1 antibodyAnti-RYDR antibody |
Information sourced from Uniprot.org