Phospho Antibodies | St John's Laboratory

Western blot analysis of lysates from PC-12 cells, using Phospho-Tyrosine Hydroxylase-S40 pAb (STJ11101143) at 1:1000 dilution or Tyrosine Hydroxylase antibody (STJ29777). PC-12 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-TH-S40 antibody (STJ11101143)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

€161.88 - €803.13

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Western blot analysis of various cells using Phospho-TH (S19) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-TH-Ser19 antibody (10-59 aa) (STJ91023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot of PC-12 cell lysate incubated in the absence (-) and presence (+) of okadaic acid (OA, 1 µM for 60 min) showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase phosphorylated at Ser31.

Anti-Phospho-TH-Ser31 antibody (STJA0003818)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat/Non-Human Primates

€568.13

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Western blot of recombinant phospho-TH and non-phospho-TH showing selective immunolabeling by the phosphospecific antibody of the ~60 kDa TH phosphorylated at Ser40. The pan-specific antibody (anti-pan-TH) recognized both the phospho-and non-phospho-TH; while most importantly, the phospho-specific antibody (anti-Ser40 TH) recognized only phospho-TH.

Anti-Phospho-TH-Ser40 antibody (STJA0003819)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ICC

Reactivity :

Many Mammalian/Many Non-mammalian

€568.13

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Western blot analysis of 293 cells using Phospho-TH (S62) Polyclonal Antibody

Anti-Phospho-TH-Ser62 antibody (1-50 aa) (STJ90748)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

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Western blot analysis of various cells using Phospho-TH (S71) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-TH-Ser71 antibody (41-90 aa) (STJ90749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of 293T 3T3 lysis using Phospho-TH (S8) antibody. Antibody was diluted at 1:1000

Anti-Phospho-TH-Ser8 antibody (2-51 aa) (STJ90837)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Rat

€60.00 - €315.00

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Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using TR-beta1 (Phospho-Ser142) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-THRB-Ser142 antibody (116-165 aa) (STJ90639)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western Blot analysis of 1 K-562 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-TIE1-Tyr1117 antibody (STJA0009945)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from TIFA knockout (KO) 293T cells transfected with TIFA using Phospho-TIFA-T9 Rabbit pAb (STJ11107723) at 1:1000 dilution incubated overnight at 4C. TIFA knockout (KO) 293T cells transfected with TIFA were treated by ADP-heptose. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 30 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.

Anti-Phospho-TIFA-T9 antibody (STJ11107723)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Western Blot analysis of cell lysis treated or untreated by LPS 100ng/mL 30min, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-TIFA-Thr9 antibody (STJ198347)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of K562 using p-TIRAP (Y86) antibody.

Anti-Phospho-TIRAP-Tyr86 antibody (52-101 aa) (STJ91272)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from HeLa cells treated with paclitaxel 1uM 24h, using TK (Phospho-Ser13) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-TK1-Ser13 antibody (1-50 aa) (STJ90834)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Pig

€60.00 - €315.00

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Anti-Phospho-TLK1-Ser764 antibody (STJA0009823)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-Phospho-TLN1-Ser-425 antibody (STJA0005613)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

€459.38

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Anti-Phospho-TLN1-Ser425 antibody (STJ196447)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Phospho-TLR3-Tyr759 antibody (STJA0009953)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Phospho-TMEM173-Ser366 antibody [PT1380R] (STJA0040278)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Mouse

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Anti-Phospho-TNFRSF21-Ser562 antibody (STJ196287)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Phospho-TNK1-Tyr277 antibody (STJ196454)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ACK1 (Phospho-Tyr284) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-TNK2-Tyr284 antibody (250-299 aa) (STJ91057)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western Blot analysis of various, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-TNK2-Tyr857+Tyr858 antibody (STJA0009977)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of extracts of HepG2 cells, using Phospho-TNK2-Y284 antibody (STJ29355) at 1:1000 dilution. HepG2 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-TNK2-Y284 antibody (STJ29355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Immunohistochemistry analysis of paraffin-embedded Human heart using Phospho-Cardiac Troponin I (TNNI3) -S23/S24 Rabbit monoclonal antibody (STJ11105867) at dilution of 1:700 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-TNNI3-S23/S24 antibody [S5867RM] (STJ11105867)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human

€183.13 - €803.13

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Western blot of mouse heart lysate showing specific immunolabeling of ~25 kDa cardiac troponin I protein phosphorylated at Ser150 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely greatly decreased by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-TNNI3-Ser150 antibody (STJA0003809)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Non-Human Primates

€568.13

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Western blot of mouse heart lysate showing specific immunolabeling of the ~25 kDa cardiac troponin I protein phosphorylated at Ser23/24 in the second lane (-). Phosphospecificity is shown in the first lane (+) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-TNNI3-Ser23/24 antibody (STJA0003808)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Non-Human Primates

€568.13

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Western blot analysis of lysates from Jurkat cells, using TNNI3 (Phospho-Ser43) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-TNNI3-Ser43 antibody (11-60 aa) (STJ90883)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot of mouse heart lysate showing specific immunolabeling of the ~25 kDa cardiac troponin I protein phosphorylated at Ser43 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is greatly decreased by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-TNNI3-Ser43 antibody (STJA0003810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Non-Human Primates/Sheep/Xenopus/Zebrafish

€568.13

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Western blot analysis of lysates from mouse heart, using TNNI3 (Phospho-Thr142) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-TNNI3-Thr142 antibody (111-160 aa) (STJ90481)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of extracts from K562 cells, using Phospho-Tensin-1 (Y1326) Polyclonal Antibody.

Anti-Phospho-TNS1-Tyr1326 antibody (STJ91358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of 3T3 cells using Phospho-Tensin-2 (Y483) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-TNS2-Tyr483 antibody (STJ90428)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of JK cells using Phospho-TOB1 (S164) Polyclonal Antibody

Anti-Phospho-TOB1-Ser164 antibody (130-179 aa) (STJ90995)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using TOP2A (Phospho-Ser1106) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-TOP2A-Ser1106 antibody (1081-1130 aa) (STJ90919)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-Phospho-TOP2A-Ser1213 antibody (STJA0009837)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-Phospho-TOP2A-Ser1469 antibody (STJ196455)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of 293T 3T3 COLO using p-Topo II Alpha (S1525) antibody. Antibody was diluted at 1:1000

Anti-Phospho-TOP2A-Ser1525 antibody (1482-1531 aa) (STJ90996)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of 293T COLO using p-Topo II Alpha (T1343) antibody. Antibody was diluted at 1:2000

Anti-Phospho-TOP2A-Thr1343 antibody (1311-1360 aa) (STJ90606)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western Blot analysis of 1HeLa cell 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-TOPBP1-Ser1159 antibody (STJA0009955)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of extracts of 293T cells, using Phospho-Tp53-S15 antibody (STJ11101082) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-TP53-S15 antibody (STJ11101082)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€161.88 - €803.13

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Western blot analysis of lysates from 293T cells using Phospho-p53-S15 Rabbit pAb (STJ22433) at 1:900 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-TP53-S15 antibody (STJ22433)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of lysates from HeLa cells, using Phospho-p53-S315 Rabbit pAb (STJ22439). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-TP53-S315 antibody (STJ22439)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€161.88 - €803.13

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Western blot analysis of lysates from HT-29 cells, using Phospho-p53-S33 Rabbit pAb (STJ25927). HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-TP53-S33 antibody (STJ116382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of lysates from HT-29 cells, using Phospho-p53-S33 Rabbit mAb (STJ11102575) at 1:1000 dilution. HT-29 cells were treated by nocodazole (100 ng/mL) at 37 °C for 16 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-TP53-S33 antibody [S5MR] (STJ11102575)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€183.13 - €803.13

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Western blot analysis of lysates from HeLa cells, using Phospho-p53-S367 Rabbit pAb (STJ11102658) at 1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-TP53-S367 antibody (STJ11102658)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Western blot analysis of various lysates using Phospho-p53-S37 Rabbit pAb (STJ11101144) at 1:1000 dilution. Both 293T cells and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-TP53-S37 antibody (STJ11101144)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of lysates from 293T cells, using Phospho-p53-S376 Rabbit mAb (STJ11102578) at 1:1000 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-TP53-S376 antibody [S8MR] (STJ11102578)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€183.13 - €803.13

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p53-S392 Rabbit pAb (STJ11101001) at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37 °C for 20 hours. NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 1s.

Anti-Phospho-TP53-S392 antibody (STJ11101001)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of lysates from C6 cells, using Phospho-p53-S392 (STJ11102634) at 1:1000 dilution. C6 cells were treated by Paclitaxel (100 nM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-TP53-S392 antibody [S4MR] (STJ11102634)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

€183.13 - €803.13

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