• Western blot analysis of extracts of various cell lines, using Phospho-Smad2 (Ser465/467) /Smad3 (Ser423/425) antibody (STJ114840) at 1:1000 dilution. HeLa and C2C12 cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of C2C12 cells, using IL2 antibody (STJ230068) at 1:1000 dilution. C2C12 cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of HeLa cells, using IL2 antibody (STJ230068) at 1:1000 dilution. HeLa cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry of paraffin-embedded Rat brain using Phospho-Smad2-S465/467 + Smad3-S423/425 antibody (STJ114840) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded Human esophagus using Phospho-Smad2-S465/467 + Smad3-S423/425 antibody (STJ114840) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-Smad2-S465/467 + Smad3-S423/425 antibody (STJ114840) at dilution of 1:100 40x lens).
  • Immunofluorescence analysis of L929 cells using Phospho-Smad2-S465/467 + Smad3-S423/425 antibody (STJ114840) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using Phospho-Smad2-S465/467 + Smad3-S423/425 antibody (STJ114840) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using Phospho-Smad2-S465/467 + Smad3-S423/425 antibody (STJ114840) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-Phospho-SMAD2/SMAD3-S423/425 antibody (STJ114840)

SKU:
STJ114840

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Smad2-S465/467 + Smad3-S423/425 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic phosphorylated peptide around S465 & S467 of human Phospho-Smad2-S465/467 + Smad3-S423/425SMAD2 (NP_005892.1).
Immunogen Sequence: SPSVRCSSMS
Background This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms.

Information sourced from Uniprot.org

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