• Western blot analysis of lysates from HT29 cells, using Smad3 (Phospho-Ser213) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-SMAD3-Ser213 antibody (179-228 aa) (STJ90524)

SKU:
STJ90524

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Mothers against decapentaplegic homolog 3-Ser213 (179-228 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMAD3
Gene ID: 4088
Uniprot ID: SMAD3_HUMAN
Immunogen Region: 179-228 aa
Specificity: Phospho-Smad3 (S213) Polyclonal Antibody detects endogenous levels of Smad3 protein only when phosphorylated at S213.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Smad3 around the phosphorylation site of Ser213 at the amino acid range 179-228
Function Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Protein Name Mothers Against Decapentaplegic Homolog 3
Mad Homolog 3
Mad3
Mothers Against Dpp Homolog 3
Hmad-3
Jv15-2
Smad Family Member 3
Smad 3
Smad3
Hsmad3
Database Links Reactome: R-HSA-1181150
Reactome: R-HSA-1502540
Reactome: R-HSA-2173788
Reactome: R-HSA-2173789
Reactome: R-HSA-2173795
Reactome: R-HSA-2173796
Reactome: R-HSA-3304356
Reactome: R-HSA-3311021
Reactome: R-HSA-3315487
Reactome: R-HSA-3656532
Reactome: R-HSA-5689880
Reactome: R-HSA-8941855
Reactome: R-HSA-8952158
Reactome: R-HSA-9008059
Reactome: R-HSA-9013695
Reactome: R-HSA-9615017
Reactome: R-HSA-9617828
Reactome: R-HSA-9735871
Reactome: R-HSA-9754189
Reactome: R-HSA-9796292
Reactome: R-HSA-9823730
Cellular Localisation Cytoplasm
Nucleus
Cytoplasmic And Nuclear In The Absence Of Tgf-Beta
On Tgf-Beta Stimulation
Migrates To The Nucleus When Complexed With Smad4
Through The Action Of The Phosphatase Ppm1a
Released From The Smad2/Smad4 Complex
And Exported Out Of The Nucleus By Interaction With Ranbp1
Co-Localizes With Lemd3 At The Nucleus Inner Membrane
Mapk-Mediated Phosphorylation Appears To Have No Effect On Nuclear Import
Pdpk1 Prevents Its Nuclear Translocation In Response To Tgf-Beta
Localized Mainly To The Nucleus In The Early Stages Of Embryo Development With Expression Becoming Evident In The Cytoplasm Of The Inner Cell Mass At The Blastocyst Stage
Alternative Antibody Names Anti-Mothers Against Decapentaplegic Homolog 3 antibody
Anti-Mad Homolog 3 antibody
Anti-Mad3 antibody
Anti-Mothers Against Dpp Homolog 3 antibody
Anti-Hmad-3 antibody
Anti-Jv15-2 antibody
Anti-Smad Family Member 3 antibody
Anti-Smad 3 antibody
Anti-Smad3 antibody
Anti-Hsmad3 antibody
Anti-SMAD3 antibody
Anti-MADH3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance