• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using SMC1 (Phospho-Ser966) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from HUVEC cells treated with etoposide 24uM 24h, using SMC1 (Phospho-Ser966) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of K562 using p-SMC1 (S966) antibody.

Anti-Phospho-SMC1A-Ser966 antibody (932-981 aa) (STJ90831)

SKU:
STJ90831

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Structural maintenance of chromosomes protein 1A-Ser966 (932-981 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMC1A
Gene ID: 8243
Uniprot ID: SMC1A_HUMAN
Immunogen Region: 932-981 aa
Specificity: Phospho-SMC1 (S966) Polyclonal Antibody detects endogenous levels of SMC1 protein only when phosphorylated at S966.
Immunogen: The antiserum was produced against synthesized peptide derived from the human SMC1 around the phosphorylation site of Ser966 at the amino acid range 932-981
Function Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
Protein Name Structural Maintenance Of Chromosomes Protein 1a
Smc Protein 1a
Smc-1-Alpha
Smc-1a
Sb1.8
Database Links Reactome: R-HSA-1221632
Reactome: R-HSA-2467813
Reactome: R-HSA-2468052
Reactome: R-HSA-2470946
Reactome: R-HSA-2500257
Reactome: R-HSA-3108214
Reactome: R-HSA-9018519
Cellular Localisation Nucleus
Chromosome
Centromere
Kinetochore
Associates With Chromatin
Before Prophase It Is Scattered Along Chromosome Arms
During Prophase
Most Of Cohesin Complexes Dissociate From Chromatin Probably Because Of Phosphorylation By Plk
Except At Centromeres
Where Cohesin Complexes Remain
At Anaphase
The Rad21 Subunit Of The Cohesin Complex Is Cleaved
Leading To The Dissociation Of The Complex From Chromosomes
Allowing Chromosome Separation
In Germ Cells
Cohesin Complex Dissociates From Chromatin At Prophase I
And May Be Replaced By A Meiosis-Specific Cohesin Complex
The Phosphorylated Form On Ser-957 And Ser-966 Associates With Chromatin During G1/S/G2 Phases But Not During M Phase
Suggesting That Phosphorylation Does Not Regulate Cohesin Function
Integral Component Of The Functional Centromere-Kinetochore Complex At The Kinetochore Region During Mitosis
Alternative Antibody Names Anti-Structural Maintenance Of Chromosomes Protein 1a antibody
Anti-Smc Protein 1a antibody
Anti-Smc-1-Alpha antibody
Anti-Smc-1a antibody
Anti-Sb1.8 antibody
Anti-SMC1A antibody
Anti-DXS423E antibody
Anti-KIAA0178 antibody
Anti-SB1.8 antibody
Anti-SMC1 antibody
Anti-SMC1L1 antibody

Information sourced from Uniprot.org

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