Anti-Phospho-SNAP25-Ser187 antibody (STJA0003778)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic phospho-peptide corresponding to amino acid residues surrounding Ser187 conjugated to KLH.
General Information
| Short Description | Rabbit polyclonal anti-Phospho-SNAP25-Ser187 for use in WB in Mouse, Rat, Guinea Pig, Human, Non-Human Primates and Zebrafish samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Mouse/Rat/Guinea Pig/Human/Non-Human Primates/Zebrafish |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | This antibody was antigen affinity purified from pooled serum. |
| Dilution Range | WB 1:1000 |
| Formulation | 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Snap25 |
| Gene ID | 25012 |
| Uniprot ID | SNP25_RAT |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser187 conjugated to KLH. |
Additional Info
| Post Translational Modifications | Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type A (BoNT/A, botA) which hydrolyzes the 197-Gln-|-Arg-198 bond and inhibits neurotransmitter release. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type E (BoNT/E) which hydrolyzes the 180-Arg-|-Ile-181 bond and inhibits neurotransmitter release. |
| Function | t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells. |
| Protein Name | Synaptosomal-Associated Protein 25Snap-25Super ProteinSupSynaptosomal-Associated 25 Kda Protein |
| Database Links | Reactome: R-RNO-181429Reactome: -RNO-181430Reactome: -RNO-210500Reactome: -RNO-212676Reactome: -RNO-264642Reactome: -RNO-449836Reactome: -RNO-6798695Reactome: -RNO-888590 |
| Cellular Localisation | CytoplasmPerinuclear RegionCell MembraneLipid-AnchorSynapseSynaptosomePhotoreceptor Inner SegmentMembrane Association Requires PalmitoylationExpressed Throughout CytoplasmConcentrating At The Perinuclear RegionColocalizes With Kcnb1 At The Cell MembraneColocalizes With Plcl1 At The Cell Membrane |
| Alternative Antibody Names | Anti-Synaptosomal-Associated Protein 25 antibodyAnti-Snap-25 antibodyAnti-Super Protein antibodyAnti-Sup antibodyAnti-Synaptosomal-Associated 25 Kda Protein antibodyAnti-Snap25 antibodyAnti-Snap antibody |
Information sourced from Uniprot.org