• Western blot of rat hippocampal lysate showing specific immunolabeling of the ~25 kDa SNAP25 phosphorylated at Ser187 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase ( Lambda-Ptase, 400 units/100ul lysate for 30 minutes, RT).

Anti-Phospho-SNAP25-Ser187 antibody (STJA0003778)

SKU:
STJA0003778-100

£442.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat/Guinea Pig/Human/Non-Human Primates/Zebrafish
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-SNAP25-Ser187 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Snap25
Gene ID: 25012
Uniprot ID: SNP25_RAT
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser187 conjugated to KLH.
Post Translational Modifications Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type A (BoNT/A, botA) which hydrolyzes the 197-Gln-|-Arg-198 bond and inhibits neurotransmitter release. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type E (BoNT/E) which hydrolyzes the 180-Arg-|-Ile-181 bond and inhibits neurotransmitter release.
Function t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
Protein Name Synaptosomal-Associated Protein 25
Snap-25
Super Protein
Sup
Synaptosomal-Associated 25 Kda Protein
Database Links Reactome: R-RNO-181429
Reactome: -RNO-181430
Reactome: -RNO-210500
Reactome: -RNO-212676
Reactome: -RNO-264642
Reactome: -RNO-449836
Reactome: -RNO-6798695
Reactome: -RNO-888590
Cellular Localisation Cytoplasm
Perinuclear Region
Cell Membrane
Lipid-Anchor
Synapse
Synaptosome
Photoreceptor Inner Segment
Membrane Association Requires Palmitoylation
Expressed Throughout Cytoplasm
Concentrating At The Perinuclear Region
Colocalizes With Kcnb1 At The Cell Membrane
Colocalizes With Plcl1 At The Cell Membrane
Alternative Antibody Names Anti-Synaptosomal-Associated Protein 25 antibody
Anti-Snap-25 antibody
Anti-Super Protein antibody
Anti-Sup antibody
Anti-Synaptosomal-Associated 25 Kda Protein antibody
Anti-Snap25 antibody
Anti-Snap antibody

Information sourced from Uniprot.org

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