Anti-Phospho-SMAD3-Ser204 antibody (170-219 aa) (STJ90523)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Smad3 around the phosphorylation site of Ser204 at the amino acid range 170-219
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Mothers against decapentaplegic homolog 3-Ser204 (170-219 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SMAD3 |
| Gene ID | 4088 |
| Uniprot ID | SMAD3_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Smad3 around the phosphorylation site of Ser204 at the amino acid range 170-219 |
| Immunogen Region | 170-219 aa |
| Specificity | Phospho-Smad3 (S204) Polyclonal Antibody detects endogenous levels of Smad3 protein only when phosphorylated at S204. |
Additional Info
| Function | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Protein Name | Mothers Against Decapentaplegic Homolog 3Mad Homolog 3Mad3Mothers Against Dpp Homolog 3Hmad-3Jv15-2Smad Family Member 3Smad 3Smad3Hsmad3 |
| Database Links | Reactome: R-HSA-1181150Reactome: R-HSA-1502540Reactome: R-HSA-2173788Reactome: R-HSA-2173789Reactome: R-HSA-2173795Reactome: R-HSA-2173796Reactome: R-HSA-3304356Reactome: R-HSA-3311021Reactome: R-HSA-3315487Reactome: R-HSA-3656532Reactome: R-HSA-5689880Reactome: R-HSA-8941855Reactome: R-HSA-8952158Reactome: R-HSA-9008059Reactome: R-HSA-9013695Reactome: R-HSA-9615017Reactome: R-HSA-9617828Reactome: R-HSA-9735871Reactome: R-HSA-9754189Reactome: R-HSA-9796292Reactome: R-HSA-9823730Reactome: R-HSA-9839394 |
| Cellular Localisation | CytoplasmNucleusCytoplasmic And Nuclear In The Absence Of Tgf-BetaOn Tgf-Beta StimulationMigrates To The Nucleus When Complexed With Smad4Through The Action Of The Phosphatase Ppm1aReleased From The Smad2/Smad4 ComplexAnd Exported Out Of The Nucleus By Interaction With Ranbp1Co-Localizes With Lemd3 At The Nucleus Inner MembraneMapk-Mediated Phosphorylation Appears To Have No Effect On Nuclear ImportPdpk1 Prevents Its Nuclear Translocation In Response To Tgf-BetaLocalized Mainly To The Nucleus In The Early Stages Of Embryo Development With Expression Becoming Evident In The Cytoplasm Of The Inner Cell Mass At The Blastocyst Stage |
| Alternative Antibody Names | Anti-Mothers Against Decapentaplegic Homolog 3 antibodyAnti-Mad Homolog 3 antibodyAnti-Mad3 antibodyAnti-Mothers Against Dpp Homolog 3 antibodyAnti-Hmad-3 antibodyAnti-Jv15-2 antibodyAnti-Smad Family Member 3 antibodyAnti-Smad 3 antibodyAnti-Smad3 antibodyAnti-Hsmad3 antibodyAnti-SMAD3 antibodyAnti-MADH3 antibody |
Information sourced from Uniprot.org