St John's Laboratory Ltd

Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-UBOX5 antibody (127-177 aa) (STJ194674)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of extracts of Rat skeletal muscle, using WWP1 antibody (STJ117813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-WWP1 antibody (1-260) (STJ117813)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Human UBE2J2 protein (Recombinant) (N-GST) (STJP014788)

Host :

E.coli

Applications :

ELISA/WB

$694.83

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Anti-NEDD4 antibody (300-350) {FITC} (STJ506278)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

$686.55

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Anti-UBE2L6 antibody [SRM14696] (STJ11109426)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of extracts of various cell lines, using USP24 antibody (STJ112599) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-USP24 antibody (2431-2620) (STJ112599)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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SDS-PAGE gel showing recombinant human Ubc6 UBE2J2 protein 3µg under reducing conditions with Coomassie blue stain

Human Ubc6/UBE2J2 protein (Recombinant) (His-Tag) (STJP017717)

Host :

E.coli

Applications :

SDS-PAGE

$185.61 - $1,019.13

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Western blot analysis of various lysates using NEDD4L Rabbit mAb (STJ11103647) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-NEDD4L antibody (876-975) [S7MR] (STJ11103647)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

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Immunofluorescence analysis of NIH/3T3 cells using UBE2L6 antibody (STJ115626) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-UBE2L6 antibody (100-153) (STJ115626)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-NEDD4 antibody (300-350) {Biotin} (STJ506277)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

$686.55

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Western blot analysis of various lysates using HERC3 Rabbit pAb (STJ111302) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-HERC3 antibody (1-200) (STJ111302)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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SDS-PAGE gel showing recombinant human UBTD2 protein stained with Coomassie blue

Human UBTD2 protein (Recombinant) (His-Tag) (STJP017806)

Host :

E.coli

Applications :

SDS-PAGE

$185.61 - $1,019.13

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Anti-Praja-1/RNF70 antibody (285-298) (STJ72775)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA

Reactivity :

Human/Mouse

$267.03

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Western blot analysis of various lysates using RNF144A Rabbit polyclonal antibody (STJ119664) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RNF144A antibody (52-179) (STJ119664)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$152.49 - $697.59

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Human UBE2S protein (Recombinant) (N-GST) (STJP014650)

Host :

E.coli

Applications :

ELISA/WB

$440.91

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Anti-CUL1 antibody (435-593) (STJ23289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunohistochemistry analysis of paraffin-embedded rat lung using Cullin 1 Rabbit polyclonal antibody (STJ11104479) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CUL1 antibody (435-593) (STJ11104479)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-Recombinant-CUL1 antibody [RM2E76] (STJA0025446)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$414.69 - $631.35

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Human CUL1 protein (Recombinant) (N-His) (STJP005350)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

$287.73 - $1,605.63

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Cullin 1 antibody western blot U-87 MG IMR-32 NIH/3T3 mouse brain STJA0033117

Anti-Cullin 1 antibody [R05-5S-8] (STJA0033117)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse

$296.01 - $425.73

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Immunohistochemistry analysis of UBE2L6 in paraffin-embedded human colon carcinoma using UBE2L6 Rabbit polyclonal antibody (STJ26021) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-UBE2L6 antibody (100-153) (STJ26021)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Immunofluorescence analysis of PC-12 cells using RBCK1 antibody (STJ11103321) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-RBCK1 antibody (60-192) (STJ11103321)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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NEDD4 antibody western blot K562 HCT116 3T3 C6 STJA0035032

Anti-NEDD4 antibody [R06-7C3] (STJA0035032)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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USP24 antibody western blot HeLa lysates STJA0035511

Anti-USP24 antibody [R43-4X-7] (STJA0035511)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Western blot analysis of various lysates using ARIH1 Rabbit polyclonal antibody (STJ11102851) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ARIH1 antibody (400 aa) (STJ11102851)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot WWP1 expression in mouse liver and PC3 cells using antibody STJA0041456

Anti-WWP1 antibody (N-Term) (STJA0041456)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat

$654.81

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Western blot analysis of various lysates using Phospho-NEDD4L-S448 Rabbit pAb (STJ11100984) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-NEDD4L-S448 antibody (STJ11100984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of various lysates using RNF166 Rabbit pAb (STJ110575) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 15s.

Anti-RNF166 antibody (102-201) (STJ110575)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various lysates using G2E3 Rabbit polyclonal antibody (STJ116620) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-G2E3 antibody (1-180) (STJ116620)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various cells using CUL-1 Polyclonal Antibody

Anti-CUL1 antibody (727-776 aa) (STJ92522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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ICC IF of UBE2L6 in HeLa cells stained with STJA0041984 antibody Alexa Fluor 488 and DAPI

Anti-UbcH8/UBE2L6 antibody (1-152aa) [k1H3] (STJA0041984)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/IHC

Reactivity :

Human

$302.91 - $458.85

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Anti-Recombinant-CUL1 antibody [RM2E77] (STJA0025447)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/WB

Reactivity :

Human/Mouse/Rat

$414.69 - $631.35

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Anti-MARCH-IV antibody (Internal) (STJ71444)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA

Reactivity :

Human

$267.03

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SDS-PAGE gel of recombinant human CuL1 protein 3μg under reducing conditions Coomassie blue stain

Human CuL1 protein (Recombinant) (His-Tag) (STJP016893)

Host :

E.coli

Applications :

SDS-PAGE

$185.61 - $1,019.13

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Western blot analysis of extracts of various cell lines, using RNF166 antibody (STJ112846) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RNF166 antibody (1-237) (STJ112846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of various lysates, using USP24 antibody (STJ11102699) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-USP24 antibody (2500-2600) [S9MR] (STJ11102699)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of various lysates using UBE2D3 Rabbit polyclonal antibody (STJ116847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-UBE2D3 antibody (1-100) (STJ116847)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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STJ73313 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Cullin 3 antibody (Internal) (STJ73313)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat

$431.25

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CUL1 antibody western blot MEF 3T3 STJA0034207

Anti-Cullin 1 antibody [R05-3D2] (STJA0034207)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Immunofluorescence analysis of PC-12 cells using USP9X/USP9Y Rabbit polyclonal antibody (STJ11103439) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-USP9Y antibody (2456-2555) (STJ11103439)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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STJ70374 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Anti-USP11/UHX1 antibody (C-Term) (STJ70374)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$431.25

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Human UBE2D1 protein (Recombinant) (N-GST) (STJP014525)

Host :

E.coli

Applications :

ELISA/WB

$440.91

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SDS-PAGE of recombinant human UbcH5a UBE2D1 protein under reducing conditions

Human UbcH5a/UBE2D1 protein (Recombinant) (His-Tag) (STJP017209)

Host :

E.coli

Applications :

SDS-PAGE

$185.61 - $1,019.13

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Immunohistochemistry analysis of paraffin-embedded human colon using UCHL3 Rabbit monoclonal antibody (STJ11101430) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-UCHL3 antibody (1-100) [S0MR] (STJ11101430)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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USP11 antibody western blot Jurkat rat brain HeLa STJA0033889

Anti-USP11 antibody [R08-2D7] (STJA0033889)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Rat

$184.23 - $388.47

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SDS-PAGE gel showing recombinant human E2-EPF UBE2S protein 3 μg under reducing conditions with Coomassie blue staining

Human E2-EPF/UBE2S protein (Recombinant) (His-Tag) (STJP016514)

Host :

E.coli

Applications :

SDS-PAGE

$152.49 - $738.99

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Human BIRC6 (Baculoviral IAP Repeat Containing Protein 6) Sandwich ELISA Kit Ready-To-Use Kit (STJE0013855)

Applications :

ELISA

Reactivity :

Human

$607.89 - $848.01

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Immunoprecipitation analysis of 200 Mu g extracts of 293T cells, using 3 Mu g UBA6 antibody (STJ29647). Western blot was performed from the immunoprecipitate using UBA6 antibody (STJ29647) at a dilution of 1:1000.

Anti-UBA6 antibody (1-260) (STJ29647)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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