• Western blot analysis of various lysates using RNF144A Rabbit polyclonal antibody (STJ119664) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RNF144A antibody (52-179) (STJ119664)

SKU:
STJ119664

€140.63 - €653.13
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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RNF144A (52-179) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNF144A
Gene ID: 9781
Uniprot ID: R144A_HUMAN
Immunogen Region: 52-179
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 52-179 of human RNF144A (NP_055561.2).
Immunogen Sequence: ELLIKEGLETAISCPDAACP KQGHLQENEIECMVAAEIMQ RYKKLQFEREVLFDPCRTWC PASTCQAVCQLQDVGLQTPQ PVQCKACRMEFCSTCKASWH PGQGCPETMPITFLPGETSA AFKMEEDD
Post Translational Modifications Autoubiquitinated.
Function E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the ubiquitination and degradation of the DNA damage kinase PRKDC.
Protein Name E3 Ubiquitin-Protein Ligase Rnf144a
Ring Finger Protein 144a
Ubcm4-Interacting Protein 4
Ubiquitin-Conjugating Enzyme 7-Interacting Protein 4
Database Links Reactome: R-HSA-8866654
Cellular Localisation Cell Membrane
Single-Pass Membrane Protein
Cytoplasmic Vesicle Membrane
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rnf144a antibody
Anti-Ring Finger Protein 144a antibody
Anti-Ubcm4-Interacting Protein 4 antibody
Anti-Ubiquitin-Conjugating Enzyme 7-Interacting Protein 4 antibody
Anti-RNF144A antibody
Anti-KIAA0161 antibody
Anti-RNF144 antibody
Anti-UBCE7IP4 antibody

Information sourced from Uniprot.org

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