• Western blot analysis of extracts of various cell lines, using UBE2J2 antibody (STJ29455) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-UBE2J2 antibody (1-230) (STJ29455)

SKU:
STJ29455

$151.88 - $705.38
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UBE2J2 (1-230) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBE2J2
Gene ID: 118424
Uniprot ID: UB2J2_HUMAN
Immunogen Region: 1-230
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human UBE2J2 (NP_477515.2).
Immunogen Sequence: MSSTSSKRAPTTATQRLKQD YLRIKKDPVPYICAEPLPSN ILEWHYVVRGPEMTPYEGGY YHGKLIFPREFPFKPPSIYM ITPNGRFKCNTRLCLSITDF HPDTWNPAWSVSTILTGLLS FMVEKGPTLGSIETSDFTKR QLAVQSLAFNLKDKVFCELF PEVVEEIKQKQKAQDELSSR PQTLPLPDVVPDGETHLVQN GIQLLNGHAPGAVPNLAGLQ QANRHHGLLG
Post Translational Modifications Auto-ubiquitinated.
Function Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD). In cooperation with the GATOR2 complex, catalyzes 'Lys-6'-linked ubiquitination of NPRL2.
Protein Name Ubiquitin-Conjugating Enzyme E2 J2
E2 Ubiquitin-Conjugating Enzyme J2
Non-Canonical Ubiquitin-Conjugating Enzyme 2
Ncube-2
Database Links Reactome: R-HSA-8866654 Q8N2K1-1
Reactome: R-HSA-9609523 Q8N2K1-1
Reactome: R-HSA-983168
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Iv Membrane Protein
Alternative Antibody Names Anti-Ubiquitin-Conjugating Enzyme E2 J2 antibody
Anti-E2 Ubiquitin-Conjugating Enzyme J2 antibody
Anti-Non-Canonical Ubiquitin-Conjugating Enzyme 2 antibody
Anti-Ncube-2 antibody
Anti-UBE2J2 antibody
Anti-NCUBE2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance