• Anti-CUL1 antibody (435-593) (STJ23289)
  • Anti-CUL1 antibody (435-593) (STJ23289)
  • Anti-CUL1 antibody (435-593) (STJ23289)
  • Immunofluorescence analysis of HeLa cells using Cullin 1 antibody (STJ23289). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using Cullin 1 Rabbit polyclonal antibody (STJ23289) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using Cullin 1 Rabbit polyclonal antibody (STJ23289) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using Cullin 1 Rabbit polyclonal antibody (STJ23289) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Cullin 1 antibody (STJ23289) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CUL1 antibody (435-593) (STJ23289)

SKU:
STJ23289

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CUL1 (435-593) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:10-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CUL1
Gene ID: 8454
Uniprot ID: CUL1_HUMAN
Immunogen Region: 435-593
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 435-593 of human Cullin 1 (NP_003583.2).
Immunogen Sequence: KNPEEAELEDTLNQVMVVFK YIEDKDVFQKFYAKMLAKRL VHQNSASDDAEASMISKLKQ ACGFEYTSKLQRMFQDIGVS KDLNEQFKKHLTNSEPLDLD FSIQVLSSGSWPFQQSCTFA LPSELERSYQRFTAFYASRH SGRKLTWLYQLSKGELVTN
Tissue Specificity Expressed in lung fibroblasts.
Post Translational Modifications Neddylated.which enhances the ubiquitination activity of SCF and prevents binding of the inhibitor CAND1. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. (Microbial infection) Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome.
Function Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(BTRC) and/or SCF(FBXW11) direct ubiquitination of CEP68. SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of CCNE1, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO1) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'.the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2.
Protein Name Cullin-1
Cul-1
Database Links Reactome: R-HSA-1169091
Reactome: R-HSA-1170546
Reactome: R-HSA-174113
Reactome: R-HSA-187577
Reactome: R-HSA-195253
Reactome: R-HSA-202424
Reactome: R-HSA-2122947
Reactome: R-HSA-2565942
Reactome: R-HSA-2644606
Reactome: R-HSA-2644607
Reactome: R-HSA-2871837
Reactome: R-HSA-2894862
Reactome: R-HSA-400253
Reactome: R-HSA-5607761
Reactome: R-HSA-5607764
Reactome: R-HSA-5610780
Reactome: R-HSA-5610783
Reactome: R-HSA-5610785
Reactome: R-HSA-5676590
Reactome: R-HSA-5684264
Reactome: R-HSA-68949
Reactome: R-HSA-69231
Reactome: R-HSA-8854050
Reactome: R-HSA-8939902
Reactome: R-HSA-8951664
Reactome: R-HSA-9020702
Reactome: R-HSA-917937
Reactome: R-HSA-9604323
Reactome: R-HSA-9708530
Reactome: R-HSA-9725371
Reactome: R-HSA-9762114
Reactome: R-HSA-983168
Alternative Antibody Names Anti-Cullin-1 antibody
Anti-Cul-1 antibody
Anti-CUL1 antibody

Information sourced from Uniprot.org

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