• Western blot analysis of extracts of various cell lines, using UBE4A antibody (STJ11102707) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of HUVEC cells, using UBE4A antibody (STJ11102707) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-UBE4A antibody (1-100) [S7MR] (STJ11102707)

SKU:
STJ11102707

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-UBE4A (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBE4A
Gene ID: 9354
Uniprot ID: UBE4A_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE4A (Q14139).
Immunogen Sequence: MTDQENNNNISSNPFAALFG SLADAKQFAAIQKEQLKQQS DELPASPDDSDNSVSESLDE FDYSVAEISRSFRSQQEICE QLNINHMIQRIFLITLDNSD
Function Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. Mediates 'Lys-48'-linked polyubiquitination of substrates.
Protein Name Ubiquitin Conjugation Factor E4 A
Ring-Type E3 Ubiquitin Transferase E4 A
Database Links Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Ubiquitin Conjugation Factor E4 A antibody
Anti-Ring-Type E3 Ubiquitin Transferase E4 A antibody
Anti-UBE4A antibody
Anti-KIAA0126 antibody

Information sourced from Uniprot.org

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