Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of HEPG2 cells using AT1 Polyclonal Antibody diluted at 1：500

Anti-AGTR1 antibody (101-150 aa) (STJ91737)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various lysates, using AT1R/AGTR1 Rabbit polyclonal antibody (STJ116133) at 1:1500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-AGTR1 antibody (294-388) (STJ116133)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-AGTR1 antibody (STJ99655)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-AGTR1 antibody [PT1438R] (STJA0040306)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Anti-AGTR1 nanobody [SAA1005] (STJN000195)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

$289.11 - $1,840.23

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Anti-AGTR1 nanobody [SAA1316] (STJN000194)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

FC/IP/RIA

Reactivity :

Human

$289.11 - $1,840.23

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Western blot analysis of lysates from Hep G2, using AGTR2 Rabbit polyclonal antibody (STJ11104644) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-AGTR2 antibody (150-250) (STJ11104644)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using AGTR2 Rabbit monoclonal antibody (STJ11101915) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-AGTR2 antibody (264-363) [S5MR] (STJ11101915)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AGTR2 antibody (30-110 aa) (STJ192611)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-AGTR2/AT2 antibody [4A03] (STJA0027403)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$351.21 - $454.71

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Anti-AGTR2/AT2 antibody [4A03] {APC} (STJA0029459)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$428.49 - $559.59

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Anti-AGTR2/AT2 antibody [4A03] {FITC} (STJA0028493)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$351.21 - $454.71

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Anti-AGTR2/AT2 antibody [4A03] {PerCP} (STJA0030425)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$403.65 - $523.71

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Anti-AGTR2/AT2 antibody [4A03] {PE} (STJA0031391)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$428.49 - $559.59

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Western blot analysis of extracts of various cell lines, using AHCYL1 antibody (STJ110084) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AHCYL1 antibody (31-260) (STJ110084)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-AHCYL1 antibody (STJA0006806)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunofluorescence analysis of HeLa cells, using AKAP12 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-AKAP12 antibody (301-350 aa) (STJ91531)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human heart tissue, using AKAP13 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-AKAP13 antibody (721-770 aa) (STJ91526)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded rat heart using AKR1C3 antibody (STJ115529) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-AKR1C3 antibody (1-323) (STJ115529)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunoprecipitation analysis of 200 Mu g extracts of K-562 cells, using 3 Mu g AKR1C3 antibody (STJ22568). Western blot was performed from the immunoprecipitate using AKR1C3 antibody (STJ22568) at a dilution of 1:1000.

Anti-AKR1C3 antibody (1-323) (STJ22568)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of lysates from HeLa and COLO cells, using AKR1C3 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-AKR1C3 antibody (191-240 aa) (STJ92671)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Confocal imaging of A549 cells using AKR1C3 Rabbit monoclonal antibody (STJ11101962, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-AKR1C3 antibody (200-300) [S2MR] (STJ11101962)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$163.53 - $719.67

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Anti-AKT antibody (STJ13100006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

$457.47

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Western blot analysis of extracts of various cell lines, using AKT1 antibody (STJ27474) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-AKT1 antibody (100-200) (STJ27474)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-Akt1 antibody (251-351 aa) [SRM5087] (STJ11106868)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $765.21

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Western blot analysis of extracts of MCF7 cells, using AKT1 antibody (STJ112889) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-AKT1 antibody (350-480) (STJ112889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Akt Mouse mAb diluted at 1:200.

Anti-AKT1 antibody (370-477 aa) [10D6] (STJ97724)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Mouse/Rat/D.melanogaster

$67.62 - $360.18

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Confocal imaging of MCF7 cells using [KO Validated] AKT1 Rabbit monoclonal antibody (STJ11104954, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-AKT1 antibody (380-480) [S4954RM] (STJ11104954)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Immunoprecipitation analysis of 300 Mu g extracts from MCF7 cells using 3 Mu g AKT1 Rabbit monoclonal antibody (STJ11101453). Western blot was performed from the immunoprecipitate using AKT1 Rabbit monoclonal antibody (STJ11101453) at a dilution of 1:1000.

Anti-AKT1 antibody (381-480) [S3MR] (STJ11101453)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

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Western blot analysis of extracts from wild type (WT) and AKT1 knockout (KO) HeLa cells, using AKT1 antibody (STJ11103457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AKT1 antibody (381-480) [S7MR] (STJ11103457)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

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Western blot analysis of lysates from 1) 3T3, 2) 293T, 3) Hela cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-AKT1 antibody (400-480 aa) (STJ91540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of human-lung tissue. 1, Akt1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-AKT1 antibody (95-144 aa) (STJ91542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemistry with Anti-AKT1 Antibody (STJ500060) at 1:50 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification. FFPE section.

Anti-AKT1 antibody (C-Term) (STJ500060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$552.69

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Western blot analysis using Akt1 monoclonal antibody against C2C12, K562 cell lysate.

Anti-AKT1 antibody (C-Term) (STJ98461)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Cow/Chicken/Pig

$205.62 - $496.80

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Anti-AKT1 antibody (C-Term) {Biotin} (STJ500061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Anti-AKT1 antibody (C-Term) {FITC} (STJ500062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Anti-AKT1 antibody (N-Term) [M101] (STJA0005251)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$440.91

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Western blot detection of AKT1 in 3T3 cell lysates using AKT1 mouse mAb (dilution 1:2000) , with super ECL.Predicted band size:60KDa.Observed band size:60KDa.

Anti-AKT1 antibody [2D9-D2-H3] (STJ99156)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Mouse/Transfected

$205.62 - $496.80

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Anti-AKT1 nanobody [SAA1080] (STJN000196)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

$289.11 - $1,840.23

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Anti-AKT1 nanobody [SAA1231] (STJN000197)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

IF

Reactivity :

Human

$289.11 - $1,840.23

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Western blot analysis of lysates from PC-3 cells using AKT1S1/PRAS40 Rabbit polyclonal antibody (STJ11104478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-AKT1S1 antibody (150-256) (STJ11104478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Immunofluorescence analysis of NIH-3T3 cells using AKT1S1/PRAS40 Rabbit polyclonal antibody (STJ27994) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-AKT1S1 antibody (150-256) (STJ27994)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using AKT1S1/AKT1S1/PRAS40 Rabbit monoclonal antibody (STJ11101861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-AKT1S1 antibody (150-256) [S1MR] (STJ11101861)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$163.53 - $719.67

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Western blot with Anti-AKT1S1 Antibody (STJ502639) of mouse liver. Antibody at 1:500 dilution in buffer.

Anti-AKT1S1 antibody (160-210) (STJ502639)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$552.69

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Anti-AKT1S1 antibody (160-210) {Biotin} (STJ502640)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Anti-AKT1S1 antibody (160-210) {FITC} (STJ502641)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Western blot analysis of various cells using PRAS40 Polyclonal Antibody

Anti-AKT1S1 antibody (207-256 aa) (STJ95215)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from 293T cells, using AKT2 Rabbit polyclonal antibody (STJ119992) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-AKT2 antibody (381-481) (STJ119992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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