Metabolism | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using Carbonic Anhydrase 1 (Carbonic Anhydrase 1 (CA1) ) antibody (STJ22854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CA1 antibody (1-261) (STJ22854)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Carbonic Anhydrase 1 (CA1) (CA1) Rabbit monoclonal antibody (STJ11102072) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CA1 antibody (160-261) [S2MR] (STJ11102072)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Carbonic Anhydrase I antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-CA1 antibody (244-261 aa) [R03-8F1] (STJA0011066)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£264.50

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Western blot analysis using CA I monoclonal antibody against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY CA1 cDNA (2).

Anti-CA1 antibody (25-90 aa) [9D6D7] (STJ97887)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using CA1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CA1 antibody (41-90 aa) (STJ91937)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of NIH-3T3 293T cells using CA XII Polyclonal Antibody diluted at 1：2000

Anti-CA12 antibody (231-280 aa) (STJ91945)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of HT-29 cells, using CA12 antibody (STJ112225) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 30s.

Anti-CA12 antibody (25-301) (STJ112225)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of CA13 in paraffin-embedded mouse kidney using CA13 Rabbit polyclonal antibody (STJ116677) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CA13 antibody (1-220) (STJ116677)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using CA XIII Polyclonal Antibody

Anti-CA13 antibody (141-190 aa) (STJ91946)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using CA14 antibody (STJ22855) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CA14 antibody (16-290) (STJ22855)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using CA XIV Polyclonal Antibody

Anti-CA14 antibody (161-210 aa) (STJ91947)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and Carbonic Anhydrase 2 (CA2) knockout (KO) 293T cells, using [KO Validated] Carbonic Anhydrase 2 (CA2) Rabbit polyclonal antibody (STJ11100007) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CA2 antibody (1-250) (STJ11100007)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using Carbonic Anhydrase 2 (CA2) antibody (STJ22856) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CA2 antibody (1-250) (STJ22856)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Carbonic Anhydrase 2 (CA2) rabbit monoclonal antibody (STJ11102438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-CA2 antibody (161-260) [S8MR] (STJ11102438)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of Mouse-kidney mouse-brain 293T hela lysis using CA II antibody. Antibody was diluted at 1:2000

Anti-CA2 antibody (180-229 aa) (STJ91938)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells using CA3 antibody (STJ115434). Blue: DAPI for nuclear staining.

Anti-CA3 antibody (1-260) (STJ115434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded rat heart using CA3 Antibody (STJ22857) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CA3 antibody (1-260) (STJ22857)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from HUVEC cells, using CA3 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-CA3 antibody (141-190 aa) (STJ91939)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using CA4 antibody (STJ22858) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CA4 antibody (19-270) (STJ22858)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of rat muscle cells using CA IV Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CA4 antibody (Internal) (STJ96493)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using CA VA Polyclonal Antibody

Anti-CA5A antibody (171-220 aa) (STJ91941)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of lysates from mouse liver using CA5A rabbit polyclonal antibody (STJ116909) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:5s.

Anti-CA5A antibody (39-305) (STJ116909)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of L929 cells using CA5B antibody (STJ119691) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CA5B antibody (1-317) (STJ119691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 3T3 cells using CA VB Polyclonal Antibody

Anti-CA5B antibody (241-290 aa) (STJ91942)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using CA VI Polyclonal Antibody

Anti-CA6 antibody (231-280 aa) (STJ91943)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using CA7 antibody (STJ116567) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CA7 antibody (1-264) (STJ116567)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CA7 antibody (STJ190654)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of Carbonic Anhydrase 9 (CA9/G250) in paraffin-embedded human esophageal using Carbonic Anhydrase 9 (CA9/G250) Rabbit monoclonal antibody (STJ11102201) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CA9 antibody (200-300) [S1MR] (STJ11102201)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of various cells using CA IX Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-CA9 antibody (33-82 aa) (STJ91940)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Carbonic Anhydrase 9 (CA9/G250) antibody (STJ111102) at 1:100 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CA9 antibody (52-151) (STJ111102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using Carbonic Anhydrase 9 (CA9/G250) antibody (STJ115637) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CA9 antibody (52-151) (STJ115637)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CA9 antibody (STJA0010190)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from 1) Hela, 2) 293T cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Tubulin Beta Polyclonal Antibody (STJ96145) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-CA9 antibody [12F10] (STJ96978)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC/IP

Reactivity :

Human

£48.00 - £252.00

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Human stomach tissue was stained with Anti-CA IX (PTR2373) Antibody

Anti-CA9 antibody [PTR2373] (STJA0006249)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/ELISA

Reactivity :

Human

£48.00 - £252.00

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Anti-CACNA1A antibody (1000-1050 aa) (STJ13100814)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-CACNA1A antibody (1401-1450 aa) (STJ192574)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-CACNA1A antibody (2000-2100 aa) (STJ11106650)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-CACNA1C antibody (1100-1180) (STJA0010115)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-CACNA1C antibody (1780-1980 aa) (STJ11107538)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-CACNA1C antibody (50-100 aa) (STJ13100816)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Anti-CACNA1C antibody (50-100 aa) (STJ13100817)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using Cav1. 2Rabbit pAb diluted at 1:200.

Anti-CACNA1C antibody (STJ197417)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using Cav1. 3Rabbit pAb diluted at 1:200.

Anti-CACNA1D antibody (1060-1140 aa) (STJ197416)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from Rat kidney, using CACNA1D Rabbit polyclonal antibody (STJ11102779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CACNA1D antibody (1882-2181) (STJ11102779)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Western blot analysis of extracts of mouse pancreas, using CACNA1D antibody (STJ117228) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5min.

Anti-CACNA1D antibody (1882-2181) (STJ117228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using CACNA1E antibody (STJ112241) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CACNA1E antibody (2014-2313) (STJ112241)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CACNA1E antibody (370-450 aa) (STJ191632)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-CACNA1E antibody (950-1000 aa) (STJ13100953)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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