Anti-ATP5A1 antibody (500-550 aa) (STJ13100022)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from aa region 500-550 of human ATP5A1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
General Information
| Short Description | Rabbit polyclonal anti-ATP5A1 (500-550 aa) for use in IHC and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1: 2000 for IHC-P and 1:3000 for WB is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | ATP5F1A |
| Gene ID | 498 |
| Uniprot ID | ATPA_HUMAN |
| Immunogen | A synthetic peptide from aa region 500-550 of human ATP5A1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse. |
| Immunogen Region | 500-550 aa |
| Specificity | Specific for ATP5A1. |
Additional Info
| Post Translational Modifications | The N-terminus is blocked. Acetylated on lysine residues. BLOC1S1 is required for acetylation. |
| Function | Subunit alpha, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (Probable). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. With the catalytic subunit beta (ATP5F1B), forms the catalytic core in the F(1) domain. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (Probable). Binds the bacterial siderophore enterobactin and can promote mitochondrial accumulation of enterobactin-derived iron ions. |
| Protein Name | Atp Synthase F(1 Complex Subunit Alpha - MitochondrialAtp Synthase F1 Subunit Alpha |
| Database Links | Reactome: R-HSA-1268020Reactome: R-HSA-163210Reactome: R-HSA-8949613Reactome: R-HSA-9837999 |
| Cellular Localisation | MitochondrionMitochondrion Inner MembranePeripheral Membrane ProteinMatrix SideCell MembraneExtracellular SideColocalizes With Hrg On The Cell Surface Of T-Cells |
| Alternative Antibody Names | Anti-Atp Synthase F(1 Complex Subunit Alpha - Mitochondrial antibodyAnti-Atp Synthase F1 Subunit Alpha antibodyAnti-ATP5F1A antibodyAnti-ATP5A antibodyAnti-ATP5A1 antibodyAnti-ATP5AL2 antibodyAnti-ATPM antibody |
Information sourced from Uniprot.org