• Western blot analysis of extracts of various cell lines, using ATP5L antibody (STJ113620) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunofluorescence analysis of PC-12 cells using ATP5L Rabbit polyclonal antibody (STJ113620) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ATP5L Rabbit polyclonal antibody (STJ113620) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of MCF7 cells using ATP5L Rabbit polyclonal antibody (STJ113620) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using ATP5L Rabbit polyclonal antibody (STJ113620) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ATP5L Rabbit polyclonal antibody (STJ113620) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ATP5L antibody (1-103) (STJ113620)

SKU:
STJ113620

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP5L (1-103) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP5MG
Gene ID: 10632
Uniprot ID: ATP5L_HUMAN
Immunogen Region: 1-103
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-103 of human ATP5L (NP_006467.4).
Immunogen Sequence: MAQFVRNLVEKTPALVNAAV TYSKPRLATFWYYAKVELVP PTPAEIPRAIQSLKKIVNSA QTGSFKQLTVKEAVLNGLVA TEVLMWFYVGEIIGKRGIIG YDV
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.
Protein Name Atp Synthase Subunit G - Mitochondrial
Atpase Subunit G
Atp Synthase Membrane Subunit G
Database Links Reactome: R-HSA-163210
Reactome: R-HSA-8949613
Cellular Localisation Mitochondrion
Mitochondrion Inner Membrane
Alternative Antibody Names Anti-Atp Synthase Subunit G - Mitochondrial antibody
Anti-Atpase Subunit G antibody
Anti-Atp Synthase Membrane Subunit G antibody
Anti-ATP5MG antibody
Anti-ATP5L antibody

Information sourced from Uniprot.org

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