| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ATIC (1-300) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ATIC |
| Gene ID: |
471 |
| Uniprot ID: |
PUR9_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ATIC (NP_004035.2). |
| Immunogen Sequence: |
MAPGQLALFSVSDKTGLVEF ARNLTALGLNLVASGGTAKA LRDAGLAVRDVSELTGFPEM LGGRVKTLHPAVHAGILARN IPEDNADMARLDFNLIRVVA CNLYPFVKTVASPGVTVEEA VEQIDIGGVTLLRAAAKNHA RVTVVCEPEDYVVVSTEMQS SESKDTSLETRRQLALKAFT HTAQYDEAISDYFRKQYSKG VSQMPLRYGMNPHQTPAQLY TLQPKLPITVLNGAPGFIN |
| Tissue Specificity | Present in the heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas. |
| Function | Bifunctional enzyme that catalyzes the last two steps of purine biosynthesis. Acts as a transformylase that incorporates a formyl group to the AMP analog AICAR (5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) to produce the intermediate formyl-AICAR (FAICAR). Can use both 10-formyldihydrofolate and 10-formyltetrahydrofolate as the formyl donor in this reaction. Also catalyzes the cyclization of FAICAR to IMP. Is able to convert thio-AICAR to 6-mercaptopurine ribonucleotide, an inhibitor of purine biosynthesis used in the treatment of human leukemias. Promotes insulin receptor/INSR autophosphorylation and is involved in INSR internalization. |
| Protein Name | Bifunctional Purine Biosynthesis Protein AticAicar Transformylase/Inosine Monophosphate CyclohydrolaseAtic Cleaved Into - Bifunctional Purine Biosynthesis Protein Atic - N-Terminally Processed Includes - Phosphoribosylaminoimidazolecarboxamide Formyltransferase5-Aminoimidazole-4-Carboxamide Ribonucleotide FormyltransferaseAicar FormyltransferaseAicar Transformylase - Inosine 5'-Monophosphate CyclohydrolaseImp CyclohydrolaseImp SynthaseInosinicase |
| Database Links | Reactome: R-HSA-73817Reactome: R-HSA-9725370 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Bifunctional Purine Biosynthesis Protein Atic antibodyAnti-Aicar Transformylase/Inosine Monophosphate Cyclohydrolase antibodyAnti-Atic Cleaved Into - Bifunctional Purine Biosynthesis Protein Atic - N-Terminally Processed Includes - Phosphoribosylaminoimidazolecarboxamide Formyltransferase antibodyAnti-5-Aminoimidazole-4-Carboxamide Ribonucleotide Formyltransferase antibodyAnti-Aicar Formyltransferase antibodyAnti-Aicar Transformylase - Inosine 5'-Monophosphate Cyclohydrolase antibodyAnti-Imp Cyclohydrolase antibodyAnti-Imp Synthase antibodyAnti-Inosinicase antibodyAnti-ATIC antibodyAnti-PURH antibodyAnti-OK antibodyAnti-SW-cl.86 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")