• Western blot analysis of extracts of various cell lines, using ATP5A1 Antibody (STJ28157) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry of paraffin-embedded rat ovary using ATP5A1 antibody (STJ28157) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human liver cancer using ATP5A1 antibody (STJ28157) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human stomach using ATP5A1 antibody (STJ28157) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse liver using ATP5A1 antibody (STJ28157) at dilution of 1:100 (40x lens).
  • Confocal immunofluorescence analysis of Hela cells using ATP5A1 antibody (STJ28157) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Confocal immunofluorescence analysis of U-2OS cells using ATP5A1 antibody (STJ28157) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 1ug ATP5A1 antibody (STJ28157). Western blot was performed from the immunoprecipitate using ATP5A1 antibody (STJ28157) at a dilition of 1:1000.

Anti-ATP5A1 antibody (1-280) (STJ28157)

SKU:
STJ28157

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP5A1 (1-280) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP5F1A
Gene ID: 498
Uniprot ID: ATPA_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATP5A1 (NP_004037.1).
Immunogen Sequence: MLSVRVAAAVVRALPRRAGL VSRNALGSSFIAARNFHASN THLQKTGTAEMSSILEERIL GADTSVDLEETGRVLSIGDG IARVHGLRNVQAEEMVEFSS GLKGMSLNLEPDNVGVVVFG NDKLIKEGDIVKRTGAIVDV PVGEELLGRVVDALGNAIDG KGPIGSKTRRRVGLKAPGII PRISVREPMQTGIKAVDSLV PIGRGQRELIIGDRQTGKTS IAIDTIINQKRFNDGSDEK
Tissue Specificity Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.
Post Translational Modifications The N-terminus is blocked. Acetylated on lysine residues. BLOC1S1 is required for acetylation.
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites. Binds the bacterial siderophore enterobactin and can promote mitochondrial accumulation of enterobactin-derived iron ions.
Protein Name Atp Synthase Subunit Alpha - Mitochondrial
Atp Synthase F1 Subunit Alpha
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-163210
Reactome: R-HSA-8949613
Cellular Localisation Mitochondrion
Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Cell Membrane
Extracellular Side
Colocalizes With Hrg On The Cell Surface Of T-Cells
Alternative Antibody Names Anti-Atp Synthase Subunit Alpha - Mitochondrial antibody
Anti-Atp Synthase F1 Subunit Alpha antibody
Anti-ATP5F1A antibody
Anti-ATP5A antibody
Anti-ATP5A1 antibody
Anti-ATP5AL2 antibody
Anti-ATPM antibody

Information sourced from Uniprot.org

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