• Western blot analysis of extracts of various cell lines, using ATPB Antibody (STJ28336) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunohistochemistry of paraffin-embedded rat heart using ATPB antibody (STJ28336) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human lung cancer using ATPB antibody (STJ28336) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human gastric cancer using ATPB antibody (STJ28336) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse heart using ATPB antibody (STJ28336) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using ATPB antibody (STJ28336) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ATPB antibody (STJ28336) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using ATPB antibody (STJ28336) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATP5B antibody (230-529) (STJ28336)

SKU:
STJ28336

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP5B (230-529) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP5F1B
Gene ID: 506
Uniprot ID: ATPB_HUMAN
Immunogen Region: 230-529
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 230-529 of human ATPB (NP_001677.2).
Immunogen Sequence: YSVFAGVGERTREGNDLYHE MIESGVINLKDATSKVALVY GQMNEPPGARARVALTGLTV AEYFRDQEGQDVLLFIDNIF RFTQAGSEVSALLGRIPSAV GYQPTLATDMGTMQERITTT KKGSITSVQAIYVPADDLTD PAPATTFAHLDATTVLSRAI AELGIYPAVDPLDSTSRIMD PNIVGSEHYDVARGVQKILQ DYKSLQDIIAILGMDELSEE DKLTVSRARKIQRFLSQPF
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Protein Name Atp Synthase Subunit Beta - Mitochondrial
Atp Synthase F1 Subunit Beta
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-163210
Reactome: R-HSA-2151201
Reactome: R-HSA-8949613
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Atp Synthase Subunit Beta - Mitochondrial antibody
Anti-Atp Synthase F1 Subunit Beta antibody
Anti-ATP5F1B antibody
Anti-ATP5B antibody
Anti-ATPMB antibody
Anti-ATPSB antibody

Information sourced from Uniprot.org

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