• Immunofluorescence analysis of U-2 OS cells using ATP5I Rabbit polyclonal antibody (STJ119174) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ATP5I in paraffin-embedded human stomach using ATP5I Rabbit polyclonal antibody (STJ119174) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ATP5I Rabbit polyclonal antibody (STJ119174) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ATP5I antibody (1-69) (STJ119174)

SKU:
STJ119174

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP5I (1-69) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP5ME
Gene ID: 521
Uniprot ID: ATP5I_HUMAN
Immunogen Region: 1-69
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-69 of human ATP5I (NP_009031.1).
Immunogen Sequence: MVPPVQVSPLIKLGRYSALF LGVAYGATRYNYLKPRAEEE RRIAAEEKKKQDELKRIARE LAEDDSILK
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.
Protein Name Atp Synthase Subunit E - Mitochondrial
Atpase Subunit E
Atp Synthase Membrane Subunit E Cleaved Into - Atp Synthase Subunit E - Mitochondrial - N-Terminally Processed
Database Links Reactome: R-HSA-163210
Reactome: R-HSA-8949613
Cellular Localisation Mitochondrion
Mitochondrion Inner Membrane
Alternative Antibody Names Anti-Atp Synthase Subunit E - Mitochondrial antibody
Anti-Atpase Subunit E antibody
Anti-Atp Synthase Membrane Subunit E Cleaved Into - Atp Synthase Subunit E - Mitochondrial - N-Terminally Processed antibody
Anti-ATP5ME antibody
Anti-ATP5I antibody
Anti-ATP5K antibody

Information sourced from Uniprot.org

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