Polyclonal Antibody | Primary Antibodies | St John's Laboratory

Western blot analysis of various lysates using Caspase-1 Rabbit pAb (STJ119181) at 1:1000 dilution incubated overnight at 4 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-CASP1 antibody (120-297) (STJ119181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Immunofluorescence analysis of Human tonsil tissue using CASP1 Rabbit polyclonal antibody (STJ11102943) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining.

Anti-CASP1 antibody (198-297) (STJ11102943)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of extracts of Mouse testis, using CASP1 antibody (STJ11103323) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CASP1 antibody (200-300) (STJ11103323)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€138.13 - €631.88

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Immunofluorescence analysis of U2OS cells using Caspase-1 Rabbit polyclonal antibody (STJ22893) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CASP1 antibody (285-404) (STJ22893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of lysates from 1) 293T, 2) Hela, 3) MCF-7, 4) Hela-UV cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Tubulin Beta monoclonal antibody (5G3) (cat: (STJ96932) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-CASP1 antibody (350-400 aa) (STJ98574)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of extracts of Jurkat, using Caspase-10 Rabbit polyclonal antibody (STJ22895) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CASP10 antibody (220-521) (STJ22895)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€138.13 - €631.88

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Western blot analysis of extracts from Jurkat, K562 cells, using Caspase-10 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CASP10 antibody (Internal) (STJ96459)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Immunohistochemistry analysis of paraffin-embedded rat spleen using Caspase-12 Rabbit polyclonal antibody (STJ112891) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CASP12 antibody (200-300) (STJ112891)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various cells using Caspase12 Polyclonal Antibody diluted at 1：500

Anti-CASP12 antibody (50-99 aa) (STJ92019)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of extracts of various cell lines, using Caspase-14 antibody (STJ28624) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CASP14 antibody (1-242) (STJ28624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various cells using Caspase-14 Polyclonal Antibody

Anti-CASP14 antibody (110-190 aa) (STJ90022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of various cells using Caspase-2 Polyclonal Antibody

Anti-CASP2 antibody (123-172 aa) (STJ92020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of extracts of various cell lines, using CASP2 Polyclonal Antibody (STJ28291) at 1:1000 dilution. Jurkat cells were treated by Staurosporine (1uM) at room temperature for 3 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CASP2 antibody (153-452) (STJ28291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various cells using Caspase-3 Polyclonal Antibody diluted at 1：1000

Anti-CASP3 antibody (116-165 aa) (STJ92021)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Fish

€61.25 - €326.25

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Western blot analysis of various lysates using [KO Validated] Caspase-3 rabbit polyclonal antibody (STJ22900) at 1:2000 dilution. Secondary antibody:HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % non-fat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-CASP3 antibody (55-277) (STJ22900)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Anti-CASP3 antibody (6-105) (STJ113073)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Immunohistochemistry analysis of Caspase-4 antibody in paraffin-embedded human lung carcinoma tissue.

Anti-CASP4 antibody (75-124 aa) (STJ92022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

€61.25 - €326.25

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Immunohistochemistry analysis of paraffin-embedded human breast cancer using Caspase-4 Rabbit polyclonal antibody (STJ11100597) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CASP4 antibody (81-270) (STJ11100597)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

€138.13 - €631.88

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STJ70593 (0. 3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CASP4 antibody (Internal) (STJ70593)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Cat/Dog/Cow

€390.63

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Western blot analysis of extracts of various cell lines, using Caspase-6 antibody (STJ22903) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CASP6 antibody (194-293) (STJ22903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€138.13 - €631.88

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Western blot analysis of various cells using Caspase-6 Polyclonal Antibody diluted at 1：1000

Anti-CASP6 antibody (223-272 aa) (STJ92023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of extracts of Rat liver, using Caspase-7 antibody (STJ22904) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Casp7 antibody (150-250) (STJ22904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute Plasma Membrane Protein Isolation and Cell Fractionation Kit (SM-005, Inventbiotech, MN, USA).

Anti-CASP7 antibody (160-240 aa) (STJ90031)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of various cells using Caspase-7 Polyclonal Antibody diluted at 1：1000

Anti-CASP7 antibody (45-94 aa) (STJ92024)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of extracts of various cell lines, using Caspase-8 antibody (STJ29787) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Casp8 antibody (376-479) (STJ29787)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Immunofluorescence analysis of HeLa cells using Caspase-8 Rabbit polyclonal antibody (STJ113762) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-Casp8 antibody (4-480) (STJ113762)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€138.13 - €631.88

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57 Yang B, Wang L, Luo X, et al. SPAG6 silencing inhibits the growth of the malignant myeloid cell lines SKM-1 and K562 via activating p53 and caspase activation-dependent apoptosis[J]. International journal of oncology, 2015, 46 (2) : 649-656.

Anti-CASP8 antibody (STJ193171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat/Pig/Chicken

€61.25 - €326.25

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CASP8AP2 antibody (1155-1205 aa) (STJ194880)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of various lysates using CASP8AP2 Rabbit polyclonal antibody (STJ118215) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CASP8AP2 antibody (1733-1982) (STJ118215)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€138.13 - €631.88

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Anti-CASP8AP2 antibody (C-Term) (STJA0005696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€610.63

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Anti-CASP8AP2 antibody (N-Term) (STJA0005695)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€610.63

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Western blot analysis of extracts of various cell lines, using Caspase-9 antibody (STJ110935) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CASP9 antibody (139-416) (STJ110935)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various lysates using Caspase-9 Rabbit pAb (STJ22908) at 1:300 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-CASP9 antibody (139-416) (STJ22908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various cells using Caspase-9 Polyclonal Antibody diluted at 1：500

Anti-CASP9 antibody (91-140 aa) (STJ92027)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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STJ70944 (1µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Caspase 3 antibody (Internal) (STJ70944)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/ICC

Reactivity :

Human/Mouse/Rat/Dog

€390.63

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STJ73021 (0. 5µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Caspase 6 antibody (Internal) (STJ73021)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

€390.63

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STJ73022 (0. 3µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Caspase 6 antibody (Internal) (STJ73022)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

€390.63

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Anti-caspase-1 antibody (C-Term) (STJ16101311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P

€1,274.38

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Western blot analysis of various cells using Caspase-10 B/C Polyclonal Antibody

Anti-Caspase-10 B/C antibody (430-479 aa) (STJ92018)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Anti-caspase-4 antibody (N-Term) (STJ16101312)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P

€1,071.88

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Western blot analysis of lysates from NIH/3T3 cells, using Cleaved-Caspase 6 p18 Rabbit pAb (STJ11106914) at 1:500 dilution. NIH/3T3 cells were treated by staurosporine (1 uM) for 3 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Caspase-6 antibody (STJ11106914)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Anti-caspase-7 antibody (C-Term) (STJ16101313)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P

€1,071.88

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Western blot analysis of lysates from Jurkat cells, using Cleaved Caspase-9 P37 Rabbit pAb (STJ11106883) at 1:900 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Caspase-9 antibody (250-350 aa) (STJ11106883)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of lysates from THP-1 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CASQ1 antibody (322-372 aa) (STJ194071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunohistochemistry analysis of CASQ2 in paraffin-embedded mouse heart using CASQ2 Rabbit polyclonal antibody (STJ119183) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CASQ2 antibody (20-399) (STJ119183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse/Rat

€138.13 - €631.88

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Anti-CASQ2 antibody (STJA0007071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunohistochemistry analysis of paraffin-embedded rat kidney using CASR antibody (STJ22909) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CASR antibody (180-536) (STJ22909)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Immunohistochemistry with Anti-CASR Antibody (STJ500366) at 1:50 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification. FFPE section.

Anti-CASR antibody (47-69) (STJ500366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€500.63

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Polyclonal Antibodies