• Western blot analysis of extracts of various cell lines, using CASP2 Polyclonal Antibody (STJ28291) at 1:1000 dilution. Jurkat cells were treated by Staurosporine (1uM) at room temperature for 3 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CASP2 antibody (153-452) (STJ28291)

SKU:
STJ28291

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Caspase-2 (153-452) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CASP2
Gene ID: 835
Uniprot ID: CASP2_HUMAN
Immunogen Region: 153-452
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 153-452 of human Caspase-2 (NP_116764.2).
Immunogen Sequence: KLRLSTDTVEHSLDNKDGPV CLQVKPCTPEFYQTHFQLAY RLQSRPRGLALVLSNVHFTG EKELEFRSGGDVDHSTLVTL FKLLGYDVHVLCDQTAQEMQ EKLQNFAQLPAHRVTDSCIV ALLSHGVEGAIYGVDGKLLQ LQEVFQLFDNANCPSLQNKP KMFFIQACRGDETDRGVDQQ DGKNHAGSPGCEESDAGKEK LPKMRLPTRSDMICGYACLK GTAAMRNTKRGSWYIEALA
Tissue Specificity Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
Post Translational Modifications The mature protease can process its own propeptide, but not that of other caspases.
Function Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress.
Protein Name Caspase-2
Casp-2
Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 2
Nedd-2
Protease Ich-1 Cleaved Into - Caspase-2 Subunit P18 - Caspase-2 Subunit P13 - Caspase-2 Subunit P12
Database Links Reactome: R-HSA-168638
Reactome: R-HSA-205025
Reactome: R-HSA-6803207
Alternative Antibody Names Anti-Caspase-2 antibody
Anti-Casp-2 antibody
Anti-Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 2 antibody
Anti-Nedd-2 antibody
Anti-Protease Ich-1 Cleaved Into - Caspase-2 Subunit P18 - Caspase-2 Subunit P13 - Caspase-2 Subunit P12 antibody
Anti-CASP2 antibody
Anti-ICH1 antibody
Anti-NEDD2 antibody

Information sourced from Uniprot.org

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