• Immunofluorescence analysis of Human tonsil tissue using CASP1 Rabbit polyclonal antibody (STJ11102943) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of various lysates using CASP1 Rabbit polyclonal antibody (STJ11102943) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CASP1 antibody (198-297) (STJ11102943)

SKU:
STJ11102943

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CASP1 (198-297) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CASP1
Gene ID: 834
Uniprot ID: CASP1_HUMAN
Immunogen Region: 198-297
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 198-297 of human CASP1 (NP_150634.1).
Immunogen Sequence: YSVDVKKNLTASDMTTELEA FAHRPEHKTSDSTFLVFMSH GIREGICGKKHSEQVPDILQ LNAIFNMLNTKNCPSLKDKP KVIIIQACRGDSPGVVWFKD
Tissue Specificity Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
Post Translational Modifications The two subunits are derived from the precursor sequence by an autocatalytic mechanism. Ubiquitinated via 'Lys-11'-linked polyubiquitination. Deubiquitinated by USP8. Cleavage in the interdomain linker region is required to induce pyroptosis.
Function Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides. Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a pro-inflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes. Cleaves a tetrapeptide after an Asp residue at position P1. Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD. In contrast to cleavage of interleukins IL1B and IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part. Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive. In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly. Isoform Delta: Apoptosis inactive. Isoform Epsilon: Apoptosis inactive.
Protein Name Caspase-1
Casp-1
Interleukin-1 Beta Convertase
Il-1bc
Interleukin-1 Beta-Converting Enzyme
Ice
Il-1 Beta-Converting Enzyme
P45 Cleaved Into - Caspase-1 Subunit P20 - Caspase-1 Subunit P10
Database Links Reactome: R-HSA-168638
Reactome: R-HSA-448706
Reactome: R-HSA-5620971
Reactome: R-HSA-6803207
Reactome: R-HSA-844456
Reactome: R-HSA-844615
Reactome: R-HSA-844623
Reactome: R-HSA-9008059
Reactome: R-HSA-9660826
Reactome: R-HSA-9692916
Cellular Localisation Cytoplasm
Cell Membrane
Alternative Antibody Names Anti-Caspase-1 antibody
Anti-Casp-1 antibody
Anti-Interleukin-1 Beta Convertase antibody
Anti-Il-1bc antibody
Anti-Interleukin-1 Beta-Converting Enzyme antibody
Anti-Ice antibody
Anti-Il-1 Beta-Converting Enzyme antibody
Anti-P45 Cleaved Into - Caspase-1 Subunit P20 - Caspase-1 Subunit P10 antibody
Anti-CASP1 antibody
Anti-IL1BC antibody
Anti-IL1BCE antibody

Information sourced from Uniprot.org

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