Anti-CASP14 antibody (1-242) (STJ28624)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Caspase-14 (1-242) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CASP14 |
| Gene ID | 23581 |
| Uniprot ID | CASPE_HUMAN |
| Immunogen Region | 1-242 |
| Immunogen Sequence | MSNPRSLEEEKYDMSGARLA LILCVTKAREGSEEDLDALE HMFRQLRFESTMKRDPTAEQ FQEELEKFQQAIDSREDPVS CAFVVLMAHGREGFLKGEDG EMVKLENLFEALNNKNCQAL RAKPKVYIIQACRGEQRDPG ETVGGDEIVMVIKDSPQTIP TYTDALHVYSTVEGYIAYRH DQKGSCFIQTLVDVFTKRKG HILELLTEVTRRMAEAELVQ EGKARKTNPEIQSTLRKRL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-242 of human Caspase-14 (NP_036246.1). |
Additional Info
| Tissue Specificity | Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only expressed at very low levels. The p17/10 mature form is expressed in epidermis stratum corneum, the p20/p8 intermediate form in epidermis upper granular cells of the stratum granulosum. |
| Post Translational Modifications | Maturation by proteolytic processing appears to be a two-step process. The precursor is processed by KLK7 to yield the p20/p8 intermediate form which acts on the precursor to yield the p17/p10 mature form. Initially, cleavage between Ile-152 and Lys-153 has been proposed to yield the large and small subunits of the active enzyme. |
| Function | Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum. Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin. In vitro has a preference for the substrate WY-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF. Involved in processing of prosaposin in the epidermis. May be involved in retinal pigment epithelium cell barrier function. Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD. |
| Protein Name | Caspase-14Casp-14 Cleaved Into - Caspase-14 Subunit P17 - Mature Form - Caspase-14 Subunit P10 - Mature Form - Caspase-14 Subunit P20 - Intermediate Form - Caspase-14 Subunit P8 - Intermediate Form |
| Database Links | Reactome: R-HSA-6809371Reactome: R-HSA-9725554 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Caspase-14 antibodyAnti-Casp-14 Cleaved Into - Caspase-14 Subunit P17 - Mature Form - Caspase-14 Subunit P10 - Mature Form - Caspase-14 Subunit P20 - Intermediate Form - Caspase-14 Subunit P8 - Intermediate Form antibodyAnti-CASP14 antibody |
Information sourced from Uniprot.org