• Immunohistochemical analysis of paraffin-embedded Human-lung, antibody was diluted at 1:100
  • Western blot analysis of lysates from 1) 293T, 2) Hela, 3) MCF-7, 4) Hela-UV cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Tubulin Beta monoclonal antibody (5G3) (cat: (STJ96932) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
  • Immunohistochemical analysis of paraffin-embedded Human-skin, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded Human-lung, antibody was diluted at 1:100
  • Western blot analysis of 293T Hela MCF-7 Hela-UV MCF-7-UV KB-UV cells using Caspase-1 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
  • Immunohistochemical analysis of paraffin-embedded Human-skin, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1, Caspase-1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, Caspase-1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, Caspase-1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Caspase-1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of rat-lung tissue. 1, Caspase-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of rat-lung tissue. 1, Caspase-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Western blot analysis of lysates from 1) 293T, 2) Hela, 3) MCF-7, 4) Hela-UV cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Tubulin Beta monoclonal antibody (5G3) (cat: (STJ96932) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-CASP1 antibody (350-400 aa) (STJ98574)

SKU:
STJ98574

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IF/WB/IHC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Caspase-1 (350-400 aa) is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IF 1:50-200
WB 1:500-2000
IHC 1:50-300
ELISA 1:10000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CASP1
Gene ID: 834
Uniprot ID: CASP1_HUMAN
Immunogen Region: 350-400 aa
Specificity: Caspase-1 Polyclonal Antibody detects endogenous levels of Caspase-1
Immunogen: The antiserum was produced against synthesized peptide derived from the C-terminal region of human CASP1 at the amino acid range 350-400
Post Translational Modifications The two subunits are derived from the precursor sequence by an autocatalytic mechanism. Ubiquitinated via 'Lys-11'-linked polyubiquitination. Deubiquitinated by USP8. Cleavage in the interdomain linker region is required to induce pyroptosis.
Function Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides. Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a pro-inflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes. Cleaves a tetrapeptide after an Asp residue at position P1. Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD. In contrast to cleavage of interleukins IL1B and IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part. Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive. In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly. Isoform Delta: Apoptosis inactive. Isoform Epsilon: Apoptosis inactive.
Protein Name Caspase-1
Casp-1
Interleukin-1 Beta Convertase
Il-1bc
Interleukin-1 Beta-Converting Enzyme
Ice
Il-1 Beta-Converting Enzyme
P45 Cleaved Into - Caspase-1 Subunit P20 - Caspase-1 Subunit P10
Database Links Reactome: R-HSA-168638
Reactome: R-HSA-448706
Reactome: R-HSA-5620971
Reactome: R-HSA-6803207
Reactome: R-HSA-844456
Reactome: R-HSA-844615
Reactome: R-HSA-844623
Reactome: R-HSA-9008059
Reactome: R-HSA-9660826
Reactome: R-HSA-9692916
Cellular Localisation Cytoplasm
Cell Membrane
Alternative Antibody Names Anti-Caspase-1 antibody
Anti-Casp-1 antibody
Anti-Interleukin-1 Beta Convertase antibody
Anti-Il-1bc antibody
Anti-Interleukin-1 Beta-Converting Enzyme antibody
Anti-Ice antibody
Anti-Il-1 Beta-Converting Enzyme antibody
Anti-P45 Cleaved Into - Caspase-1 Subunit P20 - Caspase-1 Subunit P10 antibody
Anti-CASP1 antibody
Anti-IL1BC antibody
Anti-IL1BCE antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance